Mouse Anti-CFD Recombinant Antibody (HPAB-1074-FY)
CAT#: HPAB-1074-FY
This product is a recombinant mouse antibody that recognizes CFD. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Group enriched (ASC diff, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197766-CFD
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human CFD
- Species Reactivity
- Human
- Applications
- ELISA, IHC
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
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Good Staining In IHC
We applied this mouse recombinant antibody to our Immunohistochemistry protocols for Complement Factor D. It provided clear staining in the expected tissue distributions. The antibody was affinity purified and performed well without needing excessive optimization of blocking or concentration steps in our lab.
Sensitive ELISA Reagent
This antibody functioned excellently as a detection reagent in our ELISA for CFD. It recognized the human protein with high sensitivity, enabling us to measure levels in cell culture supernatants. The fact that it is expressed in mammalian cells likely helps it maintain proper recognition of the native structure.
Q&As
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What is the target of this antibody?
A: This recombinant mouse antibody specifically recognizes Complement Factor D (CFD), also known as Adipsin. It targets the human CFD protein. The antibody is expressed in mammalian cells to ensure proper folding and recognition of the native protein structure.
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Is this antibody suitable for Immunohistochemistry?
A: Yes, this antibody is suitable for Immunohistochemistry (IHC) as well as ELISA. It has been purified by affinity chromatography and shows>95% purity by SDS-PAGE. It is a reliable reagent for detecting CFD expression in tissues or quantifying it in solution.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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