Rabbit Anti-CSF1 Recombinant Antibody (VS3-CJ542)
CAT#: VS3-CJ542
This product is a rabbit antibody that recognizes human CSF1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1709_G12_12_cr5800dfbe06bb5_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1876_A10_31_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/76624/1769_A4_33_blue_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enhanced (ASC diff, BJ hTERT+, GAMG, HHSteC, HSkMC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000184371-CSF1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human CSF1
- Species Reactivity
- Human
- Applications
- WB, ICC, IHC, IP
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation.
Target
- Alternative Names
- MCSF; CSF-1
- Gene ID
- 1435
- UniProt ID
- P09603
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- N- and O-glycosylated. Glycosylation and proteolytic cleavage yield different soluble forms. One high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. O-glycosylated with core 1 or possibly core 8 glycans. Isoform 1 is N- and O-glycosylated. Isoform 3 is only N-glycosylated.
- Protein Refseq
- NP_000748.3; NP_757349.1; NP_757350.1
- Function
- Cytokine that plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. Required for normal male and female fertility. Promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration. Plays a role in lipoprotein clearance.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| MHH-245-F(E) | Recombinant Human Anti-human CSF1 Antibody Fab Fragment | WB, Neut, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MHH-245-S(P) | Recombinant Human Anti-human CSF1 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| NEUT-504CQ | Rat Anti-Csf1 Recombinant Antibody (clone 5A1) | Block | Rat IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-505CQ | Rat Anti-Csf1 Recombinant Antibody (clone CBL205) | WB, Neut | Rat IgG2 |
| CAT | Product Name | Application | Type |
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| NEUT-506CQ | Rat Anti-Csf1 Recombinant Antibody (clone CBL535) | WB, Neut | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0824 | Hi-Affi™ Rabbit Anti-CSF1 Recombinant Antibody (clone DS824AB) | ICC, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-029ML | Anti-Human CSF1 Recombinant Antibody (TAB-029ML) | ELISA, IHC, FC, IP, IF, FuncS | IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0532-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0532-CN) | ELISA, FC, Neut | Mouse IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0533-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0533-CN) | ELISA, FC, Neut | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0534-CN | Mouse Anti-CSF1 Recombinant Antibody (HPAB-0534-CN) | ELISA, FC, Neut | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0532-CN-S(P) | Mouse Anti-CSF1 Recombinant Antibody; scFv Fragment (HPAB-0532-CN-S(P)) | ELISA, FC, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0533-CN-S(P) | Mouse Anti-CSF1 Recombinant Antibody; scFv Fragment (HPAB-0533-CN-S(P)) | ELISA, FC, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0534-CN-S(P) | Mouse Anti-CSF1 Recombinant Antibody; scFv Fragment (HPAB-0534-CN-S(P)) | ELISA, FC, Neut | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0532-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0532-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0533-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0533-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0534-CN-F(E) | Mouse Anti-CSF1 Recombinant Antibody; Fab Fragment (HPAB-0534-CN-F(E)) | ELISA, FC, Neut | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-029ML | Afuco™ Anti-CSF1 ADCC Recombinant Antibody, ADCC Enhanced (AFC-TAB-029ML) | ELISA, IHC, FC, IP, IF, FuncS | ADCC enhanced antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
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| VS-0425-YC573 | Recombinant Anti-CSF1 Vesicular Antibody, EV Displayed (VS-0425-YC573) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
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