Mouse Anti-EGF Recombinant Antibody (ZG-0281J) (CAT#: ZG-0281J)

This product is a mouse antibody that recognizes EGF.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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We require custom production
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in WB

Figure 1 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in WB

Western blot detection of EGFR in HCC827,A549 and MDA-MB-468 cell lysates using EGFR mouse mAb(dilution 1:1000). Predicted band size:134 Kda.Observed band size:175KDa.

ICC

Figure 2 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Figure 2 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Immunocytochemistry staining of HeLa cells using EGFR mouse mAb (dilution 1:200).

IP

Figure 3 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IP

Figure 3 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IP

Immunoprecipitation analysis of Hela cell lysates using EGFR mouse mAb.

IHC

Figure 4 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Figure 4 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Immunohistochemical analysis of paraffin-embedded Lung carcinoma using EGF Receptor (3F12) mouse mAb (1/800 dilution) at the Roche Benchmark XT system.

ICC

Figure 5 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Figure 5 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in ICC

Immunocytochemistry staining of MDA-MB-468 cells fixed with 4% Paraformaldehyde and using EGFR mouse mAb (dilution 1:200).

IHC

Figure 6 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Figure 6 Mouse Anti-EGF Recombinant Antibody (ZG-0281J) in IHC

Immunohistochemical analysis of paraffin-embedded Colorectal cancer using EGF Receptor (3F12) mouse mAb (1/800 dilution). Antigen retrieval was performed by pressure cooking in citrate buffer (pH 6.0).


Specifications

  • Immunogen
  • Purified recombinant human EGFR protein fragments expressed in E.coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG
  • Specificity
  • Human EGF
  • Species Reactivity
  • Human
  • Applications
  • WB, ICC, IHC

Product Property

  • Storage
  • Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Western Blot, Immunocytochemistry, Immunohistochemistry.

Target

  • Alternative Names
  • EGF; URG; HOMG4; pro-epidermal growth factor; beta-urogastrone

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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