Human Anti-EPHA2 Recombinant Antibody (clone D2) (CAT#: VS-0623-WK75)
The antibody clone D2 was produced by immunizing with recombinant EphA2. It can be used for detection of human EPHA2 in ELISA, WB and IF staining.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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IF
Figure 1 Reactivities of EphA2-specific scFv antibodies to COS-7 cell lines overexpressing EphA2 receptors
COS-7 cells were transfected with constructs overexpressing EphA2 receptors. The receptors on the surface of nonpermeabilized cells were detected by D2 antibody. Antibody-binding was visualized by secondary Alexa Fluor 488/568 antibodies.
Block
Figure 2 D2-scFv blocks the binding of ephrin-A5 to its EphA2 receptor.
When added to a D2-scFv/EphA2 complex, ephrin-A5 runs as a monomer on a size exclusion column (dotted line) but forms a heterodimeric receptor/ligand complex when mixed with an un-complexed EphA2 (dotted line).
Specifications
- Immunogen
- Recombinant EphA2
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human EPHA2
- Species Reactivity
- Human
- Clone
- D2
- Applications
- ELISA, WB, IF, Block
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No Preservative
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- ECK; CTPA; ARCC2; CTPP1; CTRCT6
- Gene ID
- 1969
- UniProt ID
- P29317
- Long Name
- EPH receptor A2
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-083 | Human Anti-EPHA2 Recombinant Antibody (clone 1C1); Fab Fragment | ELISA, WB, FuncS | Human Fab |
| HPAB-0336-YC-F(E) | Mouse Anti-EPHA2 Recombinant Antibody; Fab Fragment (HPAB-0336-YC-F(E)) | ELISA, FC, FuncS | Mouse Fab |
| HPAB-0337-YC-F(E) | Human Anti-EPHA2 Recombinant Antibody; Fab Fragment (HPAB-0337-YC-F(E)) | ELISA, FC, FuncS | Humanized Fab |
| HPAB-0526YY-F(E) | Mouse Anti-EPHA2 Recombinant Antibody (clone A-201); Fab Fragment | ELISA, IHC | Mouse Fab |
| HPAB-0527YY-F(E) | Mouse Anti-EPHA2 Recombinant Antibody (clone A-205); Fab Fragment | ELISA, IHC | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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