Human Anti-EPHA2 Recombinant Antibody (clone D2) (CAT#: VS-0623-WK75)

The antibody clone D2 was produced by immunizing with recombinant EphA2. It can be used for detection of human EPHA2 in ELISA, WB and IF staining.


Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Published Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
IF

Figure 1 Reactivities of EphA2-specific scFv antibodies to COS-7 cell lines overexpressing EphA2 receptors

Figure 1 Reactivities of EphA2-specific scFv antibodies to COS-7 cell lines overexpressing EphA2 receptors

COS-7 cells were transfected with constructs overexpressing EphA2 receptors. The receptors on the surface of nonpermeabilized cells were detected by D2 antibody. Antibody-binding was visualized by secondary Alexa Fluor 488/568 antibodies.

Block

Figure 2 D2-scFv blocks the binding of ephrin-A5 to its EphA2 receptor.

Figure 2 D2-scFv blocks the binding of ephrin-A5 to its EphA2 receptor.

When added to a D2-scFv/EphA2 complex, ephrin-A5 runs as a monomer on a size exclusion column (dotted line) but forms a heterodimeric receptor/ligand complex when mixed with an un-complexed EphA2 (dotted line).


Specifications

  • Immunogen
  • Recombinant EphA2
  • Host Species
  • Human
  • Type
  • Human IgG
  • Specificity
  • Human EPHA2
  • Species Reactivity
  • Human
  • Clone
  • D2
  • Applications
  • ELISA, WB, IF, Block
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No Preservative
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Target

  • Alternative Names
  • ECK; CTPA; ARCC2; CTPP1; CTRCT6
  • Long Name
  • EPH receptor A2

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "EPHA2"

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-1164 Hi-Affi™ Rabbit Anti-EPHA2 Recombinant Antibody (clone DS1164AB) WB Rabbit IgG

Recombinant Antibody

CAT Product Name Application Type
HPAB-0164CQ Human Anti-EPHA2 Recombinant Antibody (clone G5) ELISA, IF, FC, FuncS, Inter Humanized IgG1
HPAB-0165CQ Mouse Anti-EPHA2 Recombinant Antibody (clone B233) ELISA, IF, FC, FuncS Mouse IgG
HPAB-0166CQ Mouse Anti-EPHA2 Recombinant Antibody (clone B208) ELISA, IF, FC, FuncS Mouse IgG
HPAB-0167CQ Human Anti-EPHA2 Recombinant Antibody (clone 3F2) ELISA, IF, FC, FuncS Human IgG
HPAB-0168CQ Human Anti-EPHA2 Recombinant Antibody (clone 1F12) ELISA, IF, FC, FuncS, Activ Human IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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