Mouse Anti-GAPDH Recombinant Antibody (clone 7E4-H6-H6)
CAT#: VS3-QX460
This product is a mouse antibody that recognizes GAPDH. The antibody can be used in applications: WB.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/752_B3_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear membrane, plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach, lower shows strong nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear positivity in keratinocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_B_8_1_selected.jpg
Normal Tissue
Figure 5 IHC staining of human kidney
Immunohistochemical staining of human kidney shows cytoplasmic and nuclear positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5197/ihc_selected.jpg
Normal Tissue
Figure 6 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/79968/170623_B_3_6_selected.jpg
Subcellular Location
Figure 7 IF staining of human cell line A549
Immunofluorescent staining of human cell line A549 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/40067/1874_F7_61_blue_red_green.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_3.jpg
Normal Tissue
Figure 9 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_8_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity: 75%-25%
Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: High
Intensity: Strong
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_5.jpg
Normal Tissue
Figure 11 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_4_6.jpg
Normal Tissue
Figure 12 Lymph node
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/61280/169039_A_7_8.jpg
RNA Expression
Figure 13 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000111640-GAPDH
❮
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Specifications
- Immunogen
- Purified recombinant human GAPDH protein fragments expressed in E. coli.
- Host Species
- Mouse
- Derivation
- Mouse
- Type
- Mouse IgM
- Specificity
- GAPDH
- Species Reactivity
- Human, Monkey, Rat, Mouse, Saccharomyces cerevisiae, Pichia pastoris, Hamster
- Clone
- 7E4-H6-H6
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
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| MHH-386-F(E) | Recombinant Human Anti-human GAPDH Antibody Fab Fragment | ELISA, WB, IP, FuncS | Fab |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-386-S(P) | Recombinant Human Anti-human GAPDH Antibody scFv Fragment | WB, FC, FuncS | scFv |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0039MZ | Recombinant Mouse Anti-Human GAPDH Antibody (clone C903M) | ELISA | Mouse antibody |
| FAMAB-0117YC | Human Anti-GAPDH Recombinant Antibody (clone lesion) | ELISA, WB | Human IgG |
| MRO-0202YJ | Mouse Anti-GAPDH Polyclonal Antibody | WB, ELISA | Mouse IgG |
| MRO-0208YJ | Rabbit Anti-GAPDH Polyclonal Antibody | WB, IF, ICC, ELISA | Rabbit IgG |
| ZG-0371J | Mouse Anti-GAPDH Recombinant Antibody (ZG-0371J) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0217MZ | Chicken Anti-GAPDH Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1398 | Hi-Affi™ Rabbit Anti-GAPDH Recombinant Antibody (clone DS1398AB) | WB, ICC, IP, FC | Rabbit IgG |
| MOR-0101-FY | Rabbit Anti-GAPDH Recombinant Antibody (clone AFY0072) | IF, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0632-CN | Mouse Anti-GAPDH Recombinant Antibody (clone 5-E10) | WB, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY108 | AbPlus™ Anti-GAPDH Magnetic Beads (VS-0424-XY108) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-1024-XY212 | Mouse Anti-NHP GAPDH Recombinant Antibody (clone 1D4) | WB, IF | Mouse IgM |
| CAT | Product Name | Application | Type |
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| VS-0125-FY39 | Mouse Anti-GAPDH (clone W25lesion) scFv-Fc Chimera | ELISA, WB | Mouse IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1224-YC534 | DrugMonitor™ Anti-GAPDH Antibody (VS-1224-YC534) | ELISA, PK/PD, ADA control | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY896 | Anti-GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2729 | Anti-Mouse GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2728 | Anti-Human GAPDH Immunohistochemistry Kit | IHC | |
| VS-0525-XY2730 | Anti-Goat GAPDH Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC435 | CytoStream™ Rabbit Anti-GAPDH Recombinant Antibody (VS13-YC435) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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