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    Human Anti-GPC3 Recombinant Antibody (clone 2G9)

    CAT#: VS-0723-CJ41

    Provided is an antibody against human GPC3. This antibody bounds specifically to glypican-3-expressing cell lines and has been tested for use in enzyme-linked immunosorbent assay, flow cytometry and immunofluorescence.

    Gene Expression
    ❮
    Figure 1 IF staining of human cell line Hep G2 Figure 2 RNA cell line category: Group enriched (HAP1, Hep G2)
    ❯

    Specifications

    • Host Species
    • Human
    • Type
    • Human IgG
    • Specificity
    • Human glypican-3 (GPC3)
    • Species Reactivity
    • Human
    • Clone
    • 2G9
    • Applications
    • ELISA, FC, IF
    • Conjugate
    • Unconjugated
    • Related Disease
    • Hepatocellular carcinoma

    Product Property

    • Purification
    • Protein A purified
    • Purity
    • >95%
    • Buffer
    • PBS
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • By dot blot analysis, this antibody recognized rhGPC3 protein with no cross-reactivity with GST protein. The binding affinity for rhGPC3 was established by ELISA at the concentration of 1 ug/ml and 0.5 ug/ml rhGPC3 protein. This antibody showed intense membrane immunofluorescence staining with a significant fraction of viable HepG2.tdTomato cells.

    Target

    • Alternative Names
    • Glypican-3
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    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Datasheet

    Datasheet - VS-0723-CJ41.pdf

    MSDS

    MSDS - VS-0723-CJ41.pdf

    COA

    Certificate of Analysis Lookup

    To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

    Lot Number:

    Protocol & Troubleshooting

    Immunofluorescence (IF)

    We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

    Isotype Control

    • CAT
    • Product Name

    Secondary Antibody

    • CAT
    • Product Name

    Recommended Dilution Buffer

    • CAT
    • Product Name

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