Mouse Anti-GPC3 Recombinant Antibody (clone YP9.1) (CAT#: FAMAB-0558WJ)
This product is a mouse monoclonal antibody that is specific for human GPC3. This antibody can be used in a variety of applications, such as ELISA, WB.
Specific Inquiry
FC
Figure 1 Flow cytometry analysis of five selected mouse hybridoma clones (YP6, YP7, YP8, YP9 and YP9.1) (solid lines) and 1G12, a control anti-GPC3 mAb (dotted lines, in the middle) using A431/G1 and Hep3B.
Filled peak: isotype control.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
ELISA
Figure 2 ELISA analysis of five hybridoma clones on recombinant GPC3 proteins.
GPC3: recombinant GPC3-human Fc; GPC3ΔHS: recombinant GPC3 without HS; control: BSA. 1G12: a commercial anti-GPC3 mAb.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 3 Binding of immunotoxins on G1 cells.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 4 Cytotoxicity of immunotoxins on G1 cells. IT, immunotoxin.
The numbers in parentheses are EC50 values of immunotoxins in ng/ml.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 5 The affinity and cytotoxicity measurement of anti-GPC3 immunotoxins.
The binding of immunotoxin on GPC3+ cells.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Figure 6 The affinity and cytotoxicity measurement of anti-GPC3 immunotoxins.
The cytotoxicity of immunotoxins on cells. IT, immunotoxin.
Zhang, Y. F., & Ho, M. (2016). Humanization of high-affinity antibodies targeting glypican-3 in hepatocellular carcinoma. Scientific reports, 6(1), 1-11.
Specifications
- Immunogen
- A C-terminal peptide derived from human GPC3 isoform 2 (Residues: 511-560)
- Host Species
- Mouse
- Derivation
- Hybridoma
- Type
- Mouse IgG1, κ
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Clone
- YP9.1
- Applications
- ELISA, WB
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GPC3"
Single-domain Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NABG-069 | Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
| TAB-416CQ | Anti-Human GPC3 Therapeutic Single Domain Antibody (HN3) | IP, IHC, FuncS, WB, ELISA, Inhib | Single domain antibody |
Immunotoxin
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G035E | Anti-Gpc3 immunotoxin (scFv)-PE | Cytotoxicity assay, Function study |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-157CT | Human Anti-GPC3 Recombinant Antibody (TAB-157CT) | ELISA, FC, IHC, WB | Chimeric (mouse/human) IgG1 |
| TAB-158CT | Human Anti-GPC3 Recombinant Antibody (TAB-158CT) | ELISA, FC, IHC, WB | Chimeric (mouse/human) IgG1 |
| TAB-157CT-S(P) | Mouse Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-157CT-S(P)) | ELISA, FC, IHC, WB | Mouse scFv |
| TAB-157CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-157CT-F(E)) | ELISA, FC, IHC, WB | Chimeric (mouse/human) Fab |
| TAB-158CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-158CT-F(E)) | ELISA, FC, IHC, WB | Chimeric (mouse/human) Fab |
Human Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-167CT | Human Anti-GPC3 Recombinant Antibody (TAB-167CT) | ELISA, FC | Human IgG |
| TAB-168CT | Human Anti-GPC3 Recombinant Antibody (TAB-168CT) | ELISA, FC | Human IgG |
| TAB-167CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-167CT-F(E)) | ELISA, FC | Human Fab |
| TAB-168CT-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (TAB-168CT-F(E)) | ELISA, FC | Human Fab |
| TAB-265CQ | Anti-Human GPC3 Recombinant Antibody (MDX-1414) | ELISA, WB, FC, IHC | Human antibody |
Humanized Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-160CT-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-160CT-S(P)) | FC | Humanized scFv |
| TAB-161CT-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-161CT-S(P)) | FC | Humanized scFv |
| TAB-162CT-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-162CT-S(P)) | FC | Humanized scFv |
| TAB-163CT-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-163CT-S(P)) | FC | Humanized scFv |
| TAB-164CT-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (TAB-164CT-S(P)) | FC | Humanized scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-263CQ | Anti-Human GPC3 Recombinant Antibody (YP7) | ELISA, Inhib, IHC | |
| TAB-263CQ-S(P) | Anti-Human GPC3 Recombinant Antibody scFv Fragment (YP7) | ELISA, WB, FC, IHC | |
| TAB-263CQ-F(E) | Anti-Human GPC3 Recombinant Antibody Fab Fragment (YP7) | ELISA, WB, FC, IHC |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC033 | PE-A*02:01/Human GPC3 (FVGEFFTDV) MHC Tetramer | FCM | |
| MHC-LC034 | APC-A*02:01/Human GPC3 (FVGEFFTDV) MHC Tetramer | FCM | |
| MHC-LC035 | BV421-A*02:01/Human GPC3 (FVGEFFTDV) MHC Tetramer | FCM | |
| MHC-YF297 | A*02:01/Human GPC3 (FVGEFFTDV) MHC Monomer | MHC Multimer | |
| MHC-YF468 | H-2Kd/Human GPC3 (EYILSLEEL) MHC Pentamer | FCM |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1486 | Hi-Affi™ Rabbit Anti-GPC3 Recombinant Antibody (clone DS1486AB) | IHC-P | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0553-CN-F(E) | Human Anti-GPC3 Recombinant Antibody (clone GES1); Fab Fragment | ELISA, FC, IF, IP | Human Fab |
| HPAB-0554-CN-F(E) | Human Anti-GPC3 Recombinant Antibody (clone huG5S1); Fab Fragment | ELISA, FC, IF, IP | Humanized Fab |
| HPAB-0556-CN-F(E) | Human Anti-GPC3 Recombinant Antibody; Fab Fragment (HPAB-0556-CN-F(E)) | ELISA, WB | Human Fab |
| HPAB-0558-CN-F(E) | Mouse Anti-GPC3 Recombinant Antibody; Fab Fragment (HPAB-0558-CN-F(E)) | ELISA, WB | Mouse Fab |
| HPAB-0560-CN-F(E) | Mouse Anti-GPC3 Recombinant Antibody; Fab Fragment (HPAB-0560-CN-F(E)) | ELISA, WB | Mouse Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-1697LY-S(P) | Mouse Anti-GPC3 Recombinant Antibody (clone G5S8); scFv Fragment | ELISA, IF, Inhib, IHC | Mouse scFv |
| HPAB-1698LY-S(P) | Mouse Anti-GPC3 Recombinant Antibody (clone GES2); scFv Fragment | ELISA, IF, Inhib, IHC | Mouse scFv |
| HPAB-0689-WJ-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (HPAB-0689-WJ-S(P)) | ELISA, FC, WB | Human scFv |
| HPAB-0691-WJ-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (HPAB-0691-WJ-S(P)) | ELISA, FC, WB | Human scFv |
| HPAB-0692-WJ-S(P) | Human Anti-GPC3 Recombinant Antibody; scFv Fragment (HPAB-0692-WJ-S(P)) | ELISA, FC, WB | Human scFv |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-H14 | Afuco™ Anti-GPC3 ADCC Recombinant Antibody (Codrituzumab), ADCC Enhanced | FuncS, IF, Neut, ELISA, FC, IP | ADCC enhanced antibody |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2313LY | Human Anti-GPC3 Recombinant Antibody (HPAB-2313LY) | ELISA | Human IgG |
| HPAB-2314LY | Human Anti-GPC3 Recombinant Antibody (HPAB-2314LY) | ELISA | Human IgG |
| HPAB-2315LY | Human Anti-GPC3 Recombinant Antibody (HPAB-2315LY) | ELISA | Human IgG |
| VS3-XY776 | Mouse Anti-GPC3 Recombinant Antibody (clone 9C2) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS-0723-WK20 | Human Anti-GPC3 Recombinant Antibody (VS-0723-WK20) | ELISA | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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