Rabbit Anti-GPC3 Recombinant Antibody (ZG-0690J) (CAT#: ZG-0690J)
This product is a rabbit antibody that recognizes GPC3.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
Figure 1 Rabbit Anti-GPC3 Antibody (ZG-0690J) in WB
Western Blot
Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate
All lanes: GPC3 antibody at 0.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 60, 69 KDa
Observed band size: 66 KDa
Figure 2 Rabbit Anti-GPC3 Antibody (ZG-0690J) in IF
Immunofluorescence staining of HepG2 cells with this product at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human GPC3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GPC3
- Species Reactivity
- Human
- Applications
- ELISA, WB, IF
- Conjugate
- Uconjugated
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
WB:1:500-1:5000, IF:1:20-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "GPC3"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
NABG-069 | Recombinant Anti-Mouse Gpc3 VHH Single Domain Antibody | WB, ELISA, IHC, FuncS | Llama VHH |
TAB-416CQ | Anti-Human GPC3 Single Domain Antibody (TAB-416CQ), Research Grade Biosimilar | IP, IHC, FuncS, WB, ELISA, Inhib | Single domain antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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