Rabbit Anti-GPC3 Recombinant Antibody (ZG-0690J) (CAT#: ZG-0690J)

This product is a rabbit antibody that recognizes GPC3.

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Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

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We require custom production
  • Tested Data
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
WB

Figure 1 Rabbit Anti-GPC3 Antibody (ZG-0690J) in WB

Figure 1 Rabbit Anti-GPC3 Antibody (ZG-0690J) in WB

Western Blot
Positive WB detected in: 293 whole cell lysate, K562 whole cell lysate
All lanes: GPC3 antibody at 0.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 66, 60, 69 KDa
Observed band size: 66 KDa

IF

Figure 2 Rabbit Anti-GPC3 Antibody (ZG-0690J) in IF

Figure 2 Rabbit Anti-GPC3 Antibody (ZG-0690J) in IF

Immunofluorescence staining of HepG2 cells with this product at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).


Specifications

  • Immunogen
  • A synthesized peptide derived from human GPC3
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human GPC3
  • Species Reactivity
  • Human
  • Applications
  • ELISA, WB, IF
  • Conjugate
  • Uconjugated

Product Property

  • Purification
  • Affinity purified
  • Format
  • Liquid
  • Concentration
  • See the COA
  • Buffer
  • PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The antibody was validated for Enzyme-linked Immunosorbent Assay, Western Blot, Immunofluorescence.
    WB:1:500-1:5000, IF:1:20-1:200

Target

  • Alternative Names
  • OCI-5; Gpc3; glypican 3

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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