Rabbit Anti-GSK3A (pY216/pY279) Recombinant Antibody (clone R47-6P-9) (CAT#: VS3-WK2059)
This product is a rabbit recombinant antibody that is specific to phosphorylated GSK3A. The target of this antibody is phospho-GSK3A (Tyr216/Tyr279). The clone R47-6P-9 can be used in WB analysis at a dilution of 1/500-1/1000, IF analysis at a dilution of 1/50-1/200, IP analysis at a dilution of 1/50, and IHC staining at a dilution of 1/100-1/200. The molecular weight of GSK3A (pTyr216/pTyr279) observed in WB experiments using this antibody is 51 kDa.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/if_selected.jpg
Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28423/ihc_selected.jpg
(Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28423/ihc_selected.jpg
Figure 3 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_8.jpg
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_8.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_B_7_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_B_7_5.jpg
Figure 5 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_7_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_7_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_8_5.jpg
Figure 8 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_6_6.jpg
Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105723-GSK3A
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105723-GSK3A
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Tyr216/Tyr279 of human, mouse and rat GSK3A
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R47-6P-9
- Applications
- WB, IHC-P, ICC, IF, IP
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IHC: 1/100-1/200
IF: 1/50-1/200
IP: 1/50
Target
- Alternative Names
- Glycogen Synthase Kinase 3 Alpha; EC 2.7.11.26; Serine/Threonine-Protein Kinase GSK3A; GSK-3 Alpha; EC 2.7.11.1; EC 2.7.11
- Gene ID
- 2931
- UniProt ID
- P49840
- Long Name
- Glycogen Synthase Kinase 3 Alpha
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
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ErbB Signaling Pathway
Non-Canonical Wnt Signaling Pathway
PI3K-Akt Signaling Pathway
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Prostate Cancer
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Hepatocellular Carcinoma
Insulin Resistance
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0237MZ | Chicken Anti-GSK3a Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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