Rabbit Anti-GSK3A Recombinant Antibody (clone 4A5)
CAT#: ZG-0696J
This product is a rabbit antibody that recognizes GSK3A.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-GSK3A Antibody (ZG-0696J) in IHC
IHC image of this product diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human heart muscle
Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28423/ihc_selected.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_8.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_6_6.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105723-GSK3A
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-GSK3A/GSK3B (Y216 + Y279)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human GSK3A
- Species Reactivity
- Human
- Clone
- 4A5
- Applications
- ELISA, IHC
- Conjugate
- Uconjugated
- Related Disease
- Alzheimer's disease
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200
Target
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Related Signaling Pathways
BCR Signaling Pathway
Canonical Wnt Signaling Pathway
ErbB Signaling Pathway
Non-Canonical Wnt Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Endometrial Cancer
Prostate Cancer
Throid Cancer
Hepatocellular Carcinoma
Insulin Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| BRD-0237MZ | Chicken Anti-GSK3a Polyclonal IgY | Indirect ELISA, WB | Chicken antibody |
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| MOR-1525 | Rabbit Anti-GSK3A Recombinant Antibody (clone DS1525AB) | WB, IHC-P, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| ZG-0398J | Mouse Anti-GSK3A Recombinant Antibody (ZG-0398J) | WB, ELISA | Mouse IgG |
| ZG-0399J | Mouse Anti-GSK3A Recombinant Antibody (ZG-0399J) | WB, IF, ELISA | Mouse IgG |
| ZG-0400J | Mouse Anti-GSK3A Recombinant Antibody (ZG-0400J) | WB, IHC, ELISA | Mouse IgG |
| VS3-QX479 | Mouse Anti-GSK3A Recombinant Antibody (clone 1B10-G3-E9) | WB, IHC, ICC, FC, ELISA | |
| VS3-QX480 | Mouse Anti-GSK3A Recombinant Antibody (clone 5B8-H1-B3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0325-XY959 | Anti-GSK3A Immunohistochemistry Kit | IHC | |
| VS-0525-XY2943 | Anti-Mouse GSK3A Immunohistochemistry Kit | IHC | |
| VS-0525-XY2942 | Anti-Human GSK3A Immunohistochemistry Kit | IHC |
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