Rabbit Anti-GSK3A Recombinant Antibody (clone 4A5) (CAT#: ZG-0696J)

Name: Rabbit Anti-GSK3A Recombinant Antibody (clone 4A5)
SKU: ZG-0696J
Rating: 5 (1 reviews)

This product is a rabbit antibody that recognizes GSK3A.

Custom Production

We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

We require custom production

Tested Data
Gene Expression
Datasheet
MSDS
COA

IHC

Figure 1 Rabbit Anti-GSK3A Antibody (ZG-0696J) in IHC

Figure 1 Rabbit Anti-GSK3A Antibody (ZG-0696J) in IHC

IHC image of this product diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Subcellular Location

Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/if_selected.jpg

Normal Tissue

Figure 2 IHC staining of human heart muscle
(Immunohistochemical staining of human heart muscle shows moderate membranous and cytoplasmic positivity in myocytes.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28423/ihc_selected.jpg

Figure 3 Lymph node
(Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_8.jpg

Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_B_7_5.jpg

Figure 5 Colon
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_7_3.jpg

Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_9_4.jpg

Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_8_5.jpg

Figure 8 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4422/12766_A_6_6.jpg

RNA Expression

Figure 9 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000105723-GSK3A

Datasheet

MSDS

Certificate of Analysis Lookup
To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number

Specifications

Immunogen
A synthesized peptide derived from human Phospho-GSK3A/GSK3B (Y216 + Y279)

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human GSK3A

Species Reactivity
Human

Clone
4A5

Applications
ELISA, IHC

Conjugate
Uconjugated

Related Disease
Alzheimer's disease

Product Property

Purification
Affinity purified

Format
Liquid

Concentration
See the COA

Buffer
PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Preservative
0.02% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

Application Notes
The antibody was validated for Enzyme-linked Immunosorbent Assay, Immunohistochemistry.
IHC:1:50-1:200

Target

Alternative Names
glycogen synthase kinase-3 alpha; GSK-3 alpha; serine/threonine-protein kinase GSK3A; GSK3α

Gene ID
2931

UniProt ID
P49840

Related Resources

Related Signaling Pathways
Related Diseases

BCR Signaling Pathway

Canonical Wnt Signaling Pathway

ErbB Signaling Pathway

Non-Canonical Wnt Signaling Pathway

PI3K-Akt Signaling Pathway

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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IgG Antibody Products Chicken IgY Antibody Products Rabbit Monoclonal Antibody Products IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry

CAT	Product Name	Application	Type
MOB-2450MZ	Recombinant Mouse Anti-Human GSK3A Antibody (clone 7H3G2,7H3G3)	ELISA, IHC-Fr, IHC-P, WB	Mouse antibody
ZG-0398J	Mouse Anti-GSK3A Recombinant Antibody (ZG-0398J)	WB, ELISA	Mouse IgG
VS3-FY622	Recombinant Rabbit Anti-GSK3A Antibody (clone R07-3A2)	WB, ICC, IF, IP	Rabbit IgG
VS3-FY623	Recombinant Rabbit Anti-GSK3A Antibody (clone R08-8G7)	WB	Rabbit IgG
VS3-WK2059	Rabbit Anti-GSK3A (pY216/pY279) Recombinant Antibody (clone R47-6P-9)	WB, IHC-P, ICC, IF, IP	Rabbit IgG

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