Human Anti-GZMB Recombinant Antibody (clone Fv17) (CAT#: FAMAB-0137YC)
Granzyme B, a neutral serine protease, has been demonstrated to be a pivotal molecule for protective immunity against viral infection and cellular malignant transformation. Provided are single-chain Fv antibodies specific for granzyme B, as versatile alternatives and complementary reagents to currently available monoclonal antibodies. Owing to the availability of its DNA sequence and large scale production capability, Fv17 should be a superior reagent for monitoring granzyme B expression in natural killer cells and antigen specific CD8 T cell immunity.
Specific Inquiry
DB
Figure 1 Dot blot detection of purified granzyme B by scFvs.
Purified granzyme B (500 ng) in PBS (pH 7.4), or treated with SDS, or SDS/ -mercaptoethanol, respectively, was deposited onto nitrocellulose membranes.
IP
Figure 2 Immunoprecipitation of granzyme B by scFv and antibodies.
Immunoprecipitation of granzyme B from whole cell lysates of YTS cells.
ELISA
Figure 3 Sandwich ELISA analysis.
Fv17 was coated onto plastic wells with anti-thyroglobulin Fv as the control.
FC
Figure 4 Intracellular staining of granzyme B.
Peripheral blood mononuclear cells either unstimulated (plot A) or stimulated with influenza virus (plot B) were stained with PE-Cy7-labeled anti-CD8 and subsequently with FITC-labeled Fv17 in combination with PE-labeled anti-perforin.
Specifications
- Host Species
- Human
- Derivation
- Phage display library
- Type
- Human IgG
- Specificity
- Human GZMB
- Species Reactivity
- Human
- Clone
- Fv17
- Applications
- ELISA, FC, WB, IP
- Related Disease
- Virus infection
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- C11; HLP; CCPI; CGL1; CSPB; SECT; CGL-1; CSP-B; CTLA1; CTSGL1
- Gene ID
- 3002
- UniProt ID
- P10144
Related Resources
Allograft Rejection
Autoimmune Thyroid Disease
Graft-versus-Host Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-424 | Recombinant Anti-human GZMB Antibody | ELISA, WB, IP, FuncS | IgG |
| MHH-424 | Recombinant Human Anti-human GZMB Antibody | ELISA, IP, FuncS | IgG |
| MOB-1405MZ | Recombinant Mouse Anti-Human GZMB Antibody (clone HC22) | FC, IHC-Fr, IP | Mouse antibody |
| VS3-XY788 | Mouse Anti-GZMB Recombinant Antibody (clone 4G9) | WB, IP, IF, IHC | Mouse IgG1 |
| VS-0224-XY56 | Human Anti-GZMB Recombinant Antibody (VS-0224-XY56) | ELISA | Human IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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