Human Anti-HMGB1 Recombinant Antibody (clone G16)

CAT#: VS-0824-CJ6

G4 is a high-affinity antibody targeting High Mobility Group Box 1 (HMGB1), derived from a human Fab phage display library. G4 binds to specific regions of the HMG1 protein, including the B-Box and A-Box. The affinity of G4 was evaluated using BIAcore analysis, which showed a dissociation constant of approximately 22 nM.

Gene Expression
Figure 1 IF staining of human cell line RH-30 Figure 2 IF staining of human cell line A-431 Figure 3 IHC staining of human bone marrow Figure 4 IHC staining of human esophagus Figure 5 IF staining of human cell line U-2 OS Figure 6 IF staining of human cell line U-251 MG Figure 7 Cerebral cortex Figure 8 Colon Figure 9 Liver Figure 10 Kidney Figure 11 Lymph node Figure 12 RNA cell line category: Low cell line specificity

Specifications

  • Immunogen
  • Human HMG1 (HMGB1) protein
  • Host Species
  • Human
  • Derivation
  • Fab phage display library
  • Type
  • Human IgG
  • Specificity
  • Human HMGB1
  • Species Reactivity
  • Human
  • Clone
  • G16
  • Applications
  • ELISA
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • G4 was used in ELISA experiments to assess the binding capacity to recombinant HMG1 (rHMG1) and native HMG1.

Target

  • Alternative Names
  • HMGB1; high mobility group box 1; HMG1; HMG3; SBP-1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Secondary Antibody

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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