Human Anti-HMGB1 Recombinant Antibody (clone G16)
CAT#: VS-0824-CJ6
G4 is a high-affinity antibody targeting High Mobility Group Box 1 (HMGB1), derived from a human Fab phage display library. G4 binds to specific regions of the HMG1 protein, including the B-Box and A-Box. The affinity of G4 was evaluated using BIAcore analysis, which showed a dissociation constant of approximately 22 nM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/73663/2043_F12_32_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/617_A1_1_selected.jpg
Subcellular Location
Figure 3 IHC staining of human bone marrow
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_4_4_selected.jpg
Subcellular Location
Figure 4 IHC staining of human esophagus
Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/ihc_selected.jpg
Subcellular Location
Figure 5 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/613_A1_1_red_green.jpg
Subcellular Location
Figure 6 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5873/610_A1_1_red_green.jpg
Normal Tissue
Figure 7 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Endocrine cells
Staining: High
Intensity: Strong
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Enterocytes
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_7_5.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3506/10525_A_8_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000189403-HMGB1
❮
❯
Specifications
- Immunogen
- Human HMG1 (HMGB1) protein
- Host Species
- Human
- Derivation
- Fab phage display library
- Type
- Human IgG
- Specificity
- Human HMGB1
- Species Reactivity
- Human
- Clone
- G16
- Applications
- ELISA
- Conjugate
- Unconjugated
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- G4 was used in ELISA experiments to assess the binding capacity to recombinant HMG1 (rHMG1) and native HMG1.
Target
- Alternative Names
- HMGB1; high mobility group box 1; HMG1; HMG3; SBP-1; high mobility group protein B1; HMG-1; Amphoterin; high-mobility group box 1; high mobility group protein 1; Sulfoglucuronyl carbohydrate binding protein; high-mobility group (nonhistone chromosomal) protein 1
- Gene ID
- 3146
- UniProt ID
- P09429
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Recommended Dilution Buffer
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