Human Anti-IL10 Recombinant Antibody (clone BT-063)

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Q&As

1. Is the anti-IL10 recombinant antibody clone BT-063 suitable for use in Western blotting?

   A: Yes, the anti-IL10 recombinant antibody clone BT-063 (HPAB-0224CQ) is suitable for use in Western blotting. It provides specific binding to IL10, allowing for reliable detection in Western blot assays.

2. What are the storage recommendations for the anti-IL10 recombinant antibody clone BT-063?

   A: The recommended storage condition for the anti-IL10 recombinant antibody clone BT-063 (HPAB-0224CQ) is at -20°C or lower. For short-term storage, it can be kept at 2-8°C. To ensure stability, avoid repeated freeze-thaw cycles.

3. Can the anti-IL10 recombinant antibody clone BT-063 be used in immunoprecipitation assays?

   A: Yes, the anti-IL10 recombinant antibody clone BT-063 (HPAB-0224CQ) can be used in immunoprecipitation assays. It provides specific binding to IL10, enabling the successful precipitation of the target protein from complex mixtures.

4. Is the anti-IL10 recombinant antibody clone BT-063 effective in ELISA applications?

   A: Yes, the anti-IL10 recombinant antibody clone BT-063 (HPAB-0224CQ) is effective in ELISA applications. It has been validated for use in such assays and provides reliable detection of IL10.

5. What is the optimal dilution for using the anti-IL10 recombinant antibody clone BT-063 in immunofluorescence?

   A: The optimal dilution for using the anti-IL10 recombinant antibody clone BT-063 (HPAB-0224CQ) in immunofluorescence is typically 1:100 to 1:500. It is advisable to perform a dilution series to determine the best working concentration for your specific experimental conditions.

View the frequently asked questions answered by Creative Biolabs Support.

Citations

1. Qian, Qian, et al. "Relationship between IL-10 and PD-L1 in esophageal carcinoma tissues and IL-10 down-regulates PD-L1 expression via Met signaling pathway." Journal of Gastrointestinal Oncology 11.2 (2020): 337. https://doi.org/10.21037/jgo.2020.01.06
   The study explores the relationship between Interleukin 10 (IL-10) and Programmed Death-Ligand 1 (PD-L1) in esophageal carcinoma (ESCA) tissues, examining how IL-10 regulates PD-L1 expression via the Met signaling pathway. The research involved 100 ESCA patients who underwent surgery with preoperative therapy. The study utilized immunohistochemical staining, qRT-PCR, Western blots, and ELISA to measure PD-L1, IL-10, and Met expression levels in cancer, adjacent, and normal tissues. The findings revealed that high levels of PD-L1 and IL-10 were associated with poor prognosis in ESCA patients. The study demonstrated that IL-10 reduces PD-L1 expression in esophageal squamous cell lines through the Met signaling pathway and that the combination of crizotinib and IL-10 effectively inhibits the proliferation, invasion, and migration of these cell lines.
   Creative Biolabs contributed significantly to this research by providing the anti-IL-10 antibody used in the cell culture experiments. This antibody (CAT#: HPAB-0224CQ) was crucial for investigating the modulation of PD-L1 expression by IL-10 in esophageal squamous carcinoma cells. The provision of this antibody enabled the researchers to conduct detailed experiments on the interplay between IL-10 and PD-L1, which was essential for understanding the potential therapeutic strategies for ESCA involving immune modulation.

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(Creative Biolabs Cat# HPAB-0224CQ, RRID: AB_3111373)

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

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