Rabbit Anti-IL10 Recombinant Antibody (clone R08-3B8) (CAT#: VS3-XY3392)
The recombinant rabbit antibody is specific for IL10 (Interleukin 10), which controls the inflammatory response of macrophages by reprogramming essential metabolic pathways. IL10 targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines. The antibody specifically reacts with human IL10. It has been tested using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Normal Tissue
RNA Expression
Figure 1 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_B_9_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_B_9_5.jpg
Figure 2 Testis
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_A_6_6.jpg
(Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/71391/167603_A_6_6.jpg
Figure 3 RNA cell line category: Group enriched (HMC-1, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
Figure 3 RNA cell line category: Group enriched (HMC-1, U-266/70)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000136634-IL10
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human IL10
- Species Reactivity
- Human
- Clone
- R08-3B8
- Applications
- WB, ICC, IF, FC
- Related Disease
- Graft-Versus-Host Disease; Human Immunodeficiency Virus Type 1
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- CSIF; TGIF; GVHDS; IL-10; IL10A
- Gene ID
- 3586
- UniProt ID
- P22301
- Long Name
- Interleukin 10
- Cellular Localization
- Secreted
- Post Translation Modifications
- Glycosylation at Asn134 (NX_P22301 [NX_P22301-1])
Glycosylation from GlyGen (P22301) 1 site
- Protein Refseq
- NP_001369553.1; NP_000563.1
- Function
- Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.
Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608).
In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Asthma
Inflammatory Bowel Diseases
Systemic Lupus Erythematosus
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBW-080 | Rat Anti-IL10 Recombinant Antibody (clone 9D7); Fab Fragment | Neut | Rat Fab |
| HPAB-0223CQ-F(E) | Mouse Anti-IL10 Recombinant Antibody (clone B-N10); Fab Fragment | ELISA, FC, Block | Mouse Fab |
| HPAB-0224CQ-F(E) | Human Anti-IL10 Recombinant Antibody (clone BT-063); Fab Fragment | ELISA, FC, Block | Humanized Fab |
| HPAB-0344-WJ-F(E) | Human Anti-IL10 Recombinant Antibody (clone 12G8); Fab Fragment | ELISA | Humanized Fab |
| HPAB-0345-WJ-F(E) | Human Anti-IL10 Recombinant Antibody (clone 11D8); Fab Fragment | ELISA | Humanized Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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