Rabbit Anti-IL10 Recombinant Antibody (clone R08-3B8) (CAT#: VS3-XY3392)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human IL10

• Species Reactivity
• Human

• Clone
• R08-3B8

• Applications
• WB, ICC, IF, FC

• Related Disease
• Graft-Versus-Host Disease; Human Immunodeficiency Virus Type 1

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• WB: 1/500-1/1000
  IF: 1/50-1/200
  FC: 1/50-1/100

• Alternative Names
• CSIF; TGIF; GVHDS; IL-10; IL10A

• Gene ID
• 3586

• UniProt ID
• P22301

• Long Name
• Interleukin 10

• Cellular Localization
• Secreted

• Post Translation Modifications
• Glycosylation at Asn134 (NX_P22301 [NX_P22301-1])
  Glycosylation from GlyGen (P22301) 1 site

• Protein Refseq
• NP_001369553.1; NP_000563.1

• Function
• Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation.
  Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608).
  In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody