Mouse Anti-ITGA5 Recombinant Antibody (clone SNAKA51) (CAT#: FAMAB-1184CQ)
This product is a recombinant mouse antibody that recognizes ITGA5. The antibody was purified by affinity chromatography. It can be used in ELISA, IF.
Specific Inquiry
IHC
Figure 1 Forced expression of integrin α5 (ITGA5) markedly promotes osteogenesis in vivo.
Adult human mesenchymal stromal cells (hMSCs) transduced with LV-ITGA5 or empty vector were implanted s.c. with coral/hydroxyapatite (Co) at ectopic sites in nude mice, and bone formation was examined after 8 weeks. Histological examination showed that ITGA5 overexpression in hMSCs increased new bone formation along the carrier compared with that in osteocytes (Ocy).
Hamidouche, Z., Fromigué, O., Ringe, J., Häupl, T., Vaudin, P., Pagès, J. C., ... & Marie, P. J. (2009). Priming integrin α5 promotes human mesenchymal stromal cell osteoblast differentiation and osteogenesis. Proceedings of the National Academy of Sciences, 106(44), 18587-18591.
IF
Figure 2 Forced expression of integrin α5 (ITGA5) promotes osteoblast differentiation in human mesenchymal stromal cells (hMSCs).
Adult hMSCs were transduced with LV-ITGA5 or empty vector, the efficacy of transduction was evaluated by GFP level under fluorescence microscopy.
Hamidouche, Z., Fromigué, O., Ringe, J., Häupl, T., Vaudin, P., Pagès, J. C., ... & Marie, P. J. (2009). Priming integrin α5 promotes human mesenchymal stromal cell osteoblast differentiation and osteogenesis. Proceedings of the National Academy of Sciences, 106(44), 18587-18591.
WB
Figure 3 Forced expression of integrin α5 (ITGA5) promotes osteoblast differentiation in human mesenchymal stromal cells (hMSCs).
Adult hMSCs were transduced with LV-ITGA5 or empty vector, the efficacy of transduction was evaluated by Western blot analysis.
Hamidouche, Z., Fromigué, O., Ringe, J., Häupl, T., Vaudin, P., Pagès, J. C., ... & Marie, P. J. (2009). Priming integrin α5 promotes human mesenchymal stromal cell osteoblast differentiation and osteogenesis. Proceedings of the National Academy of Sciences, 106(44), 18587-18591.
Specifications
- Immunogen
- Human placental α5β1 integrin
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human α5 integrin subunit
- Species Reactivity
- Human
- Clone
- SNAKA51
- Applications
- IHC, WB, IF
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1727z | Mouse Anti-ITGA5 Recombinant Antibody (clone 11B1) | WB, ELISA, FC, IHC | Mouse IgG2a |
| MOB-0506CT | Recombinant Mouse anti-Human ITGA5 Monoclonal antibody (DC50f) | FC | |
| MRO-0849-CN | Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (CBACN-310) | WB, IF, IHC, IP, FC | Rabbit IgG |
| HPAB-0398-WJ | Human Anti-ITGA5 Recombinant Antibody (HPAB-0398-WJ) | ELISA | Humanized IgG |
| HPAB-0399-WJ | Mouse Anti-ITGA5 Recombinant Antibody (HPAB-0399-WJ) | FC | Mouse IgG |
Chimeric Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-077 | Anti-Human Integrin alpha 5 beta 1 Recombinant Antibody (Volociximab) | ELISA, IHC | IgG4 |
Blocking Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1554CQ | Recombinant Mouse Anti-ITGA5 Antibody (NKI-SAM-1) | FC, IP, ELISA, BL | IgG2b, κ |
| NEUT-1555CQ | Recombinant Armenian Hamster Anti-Itga5 Antibody (HMα5-1) | FC, BL, IP | IgG |
| NEUT-1556CQ | Recombinant Rat Anti-Itga5 Antibody (5H10-27) | FC, BL, IHC-Fr, FuncS | IgG2a, κ |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1874 | Hi-Affi™ Recombinant Rabbit Anti-ITGA5 Monoclonal Antibody (DS1874AB) | WB, IHC-P, ICC/IF, IP, FC | IgG |
ADCC Enhanced Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AFC-TAB-077 | Afuco™ Anti-ITGA5 ADCC Recombinant Antibody (Volociximab), ADCC Enhanced | IP, IF, FuncS, FC, Neut, ELISA | ADCC enhanced antibody |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-WJ-S(P) | Human Anti-ITGA5 Recombinant Antibody; scFv Fragment (HPAB-0398-WJ-S(P)) | ELISA | Humanized scFv |
| HPAB-0399-WJ-S(P) | Mouse Anti-ITGA5 Recombinant Antibody; scFv Fragment (HPAB-0399-WJ-S(P)) | ELISA | Mouse scFv |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0398-WJ-F(E) | Human Anti-ITGA5 Recombinant Antibody; Fab Fragment (HPAB-0398-WJ-F(E)) | ELISA | Humanized Fab |
| HPAB-0399-WJ-F(E) | Mouse Anti-ITGA5 Recombinant Antibody; Fab Fragment (HPAB-0399-WJ-F(E)) | ELISA | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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