Rabbit Anti-ITGB3 Recombinant Antibody (VS13-YC605)

Name: Rabbit Anti-ITGB3 Recombinant Antibody (VS13-YC605)
SKU: VS13-YC605
Rating: 5 (1 reviews)

CAT#: VS13-YC605

The Rabbit Anti-ITGB3 Recombinant Antibody is specific for human and mouse ITGB3. ITGB3 is the integrin beta chain beta 3, forming heterodimeric integral cell surface proteins with alpha chains for functions like cell adhesion and signaling. It pairs with the alpha IIb chain in platelets, vital for integrin-mediated processes. The anti-ITGB3 antibody can be used for Western blot (1/1000), Immunocytochemistry (1/50-1/200), Immunofluorescence (1/50-1/200), Immunohistochemistry-paraffin (1/50-1/400).

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/281_B9_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/ihc_selected.jpg

Normal Tissue Figure 3 Bone marrow

Hematopoietic cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/27852/64673_B_4_4.jpg

RNA Expression Figure 4 RNA cell line category: Cell line enhanced (EFO-21, HEL, RPTEC TERT1, U-87 MG, WM-115)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000259207-ITGB3

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Specifications

Immunogen
Recombinant human Integrin beta 3, aa 729-788.

Host Species
Rabbit

Type
Rabbit IgG

Specificity
Human and Mouse ITGB3

Species Reactivity
Human, Mouse

Applications
WB, IHC-P, ICC, IF

Conjugate
NONE

Product Property

Clonality
Monoclonal

Purification
Protein A purified

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

Preservative
0.05% Sodium azide

Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Shipping with ice pack.

Applications

Application Notes
Western blot (WB): 1/1000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/400

Target

Alternative Names
Integrin Subunit Beta 3

Gene ID
3690

UniProt ID
P05106

Long Name
Integrin Subunit Beta 3

Cellular Localization
Cell membrane; Cell projection; Cell junction

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS13-YC605.pdf

MSDS

MSDS - VS13-YC605.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

Lot Number:

Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

CAT
Product Name

Isotype Control

CAT
Product Name

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