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  • Rabbit Anti-JUN (pT91) Recombinant Antibody (clone R04-1X1)

Rabbit Anti-JUN (pT91) Recombinant Antibody (clone R04-1X1) (CAT#: VS3-WK2149)

This product is a rabbit recombinant antibody that is specific to phosphorylated JUN. The target of this antibody is phospho-JUN (Thr91). The clone R04-1X1 can be used in WB analysis at a dilution of 1/500-1/1000 and IP analysis at a dilution of 1/20. The molecular weight of JUN (pThr91) observed in WB experiments using this antibody is 43 kDa.

Specific Inquiry
  • Size:
  • Conjugation:
  • Endotoxin:
  • Purity:
  • Formats:
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  • Modalities:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31174/1313_G11_5_selected.jpg
Figure 2 IF staining of human cell line A-431
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human small intestine
Figure 3 IHC staining of human small intestine
(Immunohistochemical staining of human small intestine shows moderate nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_6_2_selected.jpg
Figure 4 IHC staining of human fallopian tube
Figure 4 IHC staining of human fallopian tube
(High expression. RNA expression: 314.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7780/18871_A_8_7_val_selected.jpg
Figure 5 IHC staining of human lymph node
Figure 5 IHC staining of human lymph node
(Low expression. RNA expression: 52.1 nTPM)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7780/18871_A_8_8_val_selected.jpg
Figure 6 Cerebral cortex
Figure 6 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_B_7_5.jpg
Figure 7 Thyroid gland
Figure 7 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_B_2_5.jpg
Figure 8 Colon
Figure 8 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_9_3.jpg
Figure 9 Liver
Figure 9 Liver
(Hepatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_7_4.jpg
Figure 10 Kidney
Figure 10 Kidney
(Distal tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Proximal tubules (cell body)
Staining: Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_8_5.jpg
Figure 11 Testis
Figure 11 Testis
(Sertoli cells
Staining: Not detected
Intensity: Weak
Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3801/10121_A_5_6.jpg
RNA Expression
Figure 12 RNA cell line category: Cell line enhanced (RH-30)
Figure 12 RNA cell line category: Cell line enhanced (RH-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000177606-JUN

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Phospho-Thr91 of human, mouse and rat JUN
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-1X1
  • Applications
  • WB, IP
  • Conjugate
  • Unconjugated

Product Property

  • Clonality
  • Monoclonal
  • Purification
  • Protein A affinity purified
  • Purity
  • >95% as determined by SDS-PAGE
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
  • Preservative
  • 0.02% Sodium azide
  • Storage
  • Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • WB: 1/500-1/1000
    IP: 1/20

Target

  • Alternative Names
  • AP1; p39; AP-1; cJUN; c-Jun
  • Long Name
  • Jun proto-oncogene, AP-1 transcription factor subunit

Recommended Products

Isotype Control

Secondary Antibody

Related Resources

  • Related Signaling Pathways
  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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