What is the function of the target protein for clone DX-2802?

The target, MMP9, is a matrix metalloproteinase involved in the breakdown of extracellular matrix in processes like tissue remodeling and metastasis. This recombinant human antibody (clone DX-2802) specifically recognizes human MMP9.

Is this antibody suitable for Flow Cytometry?

Yes, this antibody has been validated for use in Flow Cytometry (FC) as well as ELISA. It is a Human IgG1 antibody that can be used to detect MMP9 expression in various research samples.

Human Anti-MMP9 Recombinant Antibody (clone DX-2802)

CAT#: HPAB-0199-FY

This product is a recombinant human antibody that recognizes human MMP9. It may play a part in treating or preventing an inflammatory disorder.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/if_selected.jpg

Subcellular Location Figure 2 IHC staining of human bone marrow

Immunohistochemical staining of human bone marrow shows strong nuclear and cytoplasmic positivity in bone marrow poietic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/348/ihc_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/30_H1_1_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1238/31_H1_1_blue_red_green.jpg

Normal Tissue Figure 5 Testis

Preleptotene spermatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Spermatogonia cells
Staining: Medium
Intensity: Moderate
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_6_6.jpg

Normal Tissue Figure 6 Lymph node

Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63909/152375_A_8_8.jpg

RNA Expression Figure 7 RNA cell line category: Cell line enriched (NB-4)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000100985-MMP9

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Specifications

Immunogen
Recombinant human MMP9 protein

Host Species
Human

Type
Human IgG1, λ

Specificity
Human MMP9

Species Reactivity
Human

Clone
DX-2802

Applications
ELISA, FC

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Matrix Metallopeptidase 9; Matrix Metalloproteinase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); EC 3.4.24.35; CLG4B; MMP-9; GELB; Matrix Metallopeptidase 9 (Gelatinase B, 92kDa Gelatinase, 92kDa Type IV Collagenase); Matrix Metalloproteinase-9;

Gene ID
4318

UniProt ID
P14780

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Breast cancer biomarkers at key points during disease progression

Precise MMP9 Detection

We employed clone DX-2802 for detecting MMP9 in our flow cytometry panels. The antibody provided good separation and specific staining of the target cells. Knowing it is a recombinant human antibody gives us assurance regarding its specificity for human MMP9, which is crucial for our matrix metalloproteinase studies.

Works Well In ELISA

This antibody functioned perfectly in our ELISA setup for measuring MMP9. The signal-to-noise ratio was high, and the product consistency has been excellent across different orders. It has helped us generate reliable data regarding tissue remodeling enzymes in our research samples.

Q&As

What is the function of the target protein for clone DX-2802?

A: The target, MMP9, is a matrix metalloproteinase involved in the breakdown of extracellular matrix in processes like tissue remodeling and metastasis. This recombinant human antibody (clone DX-2802) specifically recognizes human MMP9.
Is this antibody suitable for Flow Cytometry?

A: Yes, this antibody has been validated for use in Flow Cytometry (FC) as well as ELISA. It is a Human IgG1 antibody that can be used to detect MMP9 expression in various research samples.

View the frequently asked questions answered by Creative Biolabs Support.

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Related Signaling Pathways

TNF Signaling Pathway

Downloadable Resources

Download resources about recombinant antibody development and antibody engineering to boost your research.

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-0199-FY.pdf

MSDS

MSDS - HPAB-0199-FY.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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