This product is a recombinant mouse antibody that recognizes MSLN. The antibody was purified by affinity chromatography. It can be used for FC and other applications.
Figure 1 Mouse Anti-Human MSLN Antibody (HPAB-0886-FY) in DB
Dot Blot analysis of HPAB-0886-FY was performed by coating with Human MSLN Protein (His Tag).
HPAB-0886-FY incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000)
Figure 2 Mouse Anti-Human MSLN Antibody (HPAB-0886-FY) in ELISA
ELISA analysis of HPAB-0886-FY was performed by coating with Human MSLN Protein (His Tag). Then blocked with BSA and incubated with HPAB-0886-FY. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1: 2000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 3 Mouse Anti-Human MSLN Antibody (HPAB-0886-FY) in WB
Western blot analysis of HPAB-0886-FY was performed by loading Human MSLN Protein (His Tag).
HPAB-0886-FY incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-mouse IgG as a secondary antibody (1: 2000)
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-1165z | Mouse Anti-MSLN Recombinant Antibody (clone 44C3) | WB, ELISA, IHC | Mouse IgG2a |
HPAB-0442LY | Human Anti-MSLN Recombinant Antibody (HPAB-0442LY) | ELISA, FC, IHC, WB | Human IgG1 |
HPAB-0443LY | Human Anti-MSLN Recombinant Antibody (HPAB-0443LY) | ELISA, FC, IHC, WB | Human IgG1 |
HPAB-0444LY | Human Anti-MSLN Recombinant Antibody (HPAB-0444LY) | ELISA, FC, IHC, WB | Human IgG1 |
HPAB-M0155-YC | Human Anti-MSLN Recombinant Antibody (clone M/YC-16) | ELISA, WB | Chimeric (mouse/human) IgG |
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# HPAB-0886-FY, RRID: AB_3111412)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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