Rabbit Anti-MTOR (pS2481) Recombinant Antibody (clone R08-9E3)
CAT#: VS3-WK2163
This product is a rabbit recombinant antibody that is specific to phosphorylated MTOR. The target of this antibody is phospho-MTOR (Ser2481). The clone R08-9E3 can be used in WB analysis at a dilution of 1/500-1/1000. The molecular weight of MTOR (pSer2481) observed in WB experiments using this antibody is 289 kDa.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/71227/1564_C3_1_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_6_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_B_9_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Hepatocytes Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/69425/153895_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000198793-MTOR
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Phospho-Ser2481 of human, mouse and rat MTOR
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R08-9E3
- Applications
- WB
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% Glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
Target
- Alternative Names
- Mechanistic Target Of Rapamycin Kinase; Rapamycin And FKBP12 Target 1; Mammalian Target Of Rapamycin; FK506-Binding Protein 12-Rapamycin Complex-Associated Protein 1; Mechanistic Target Of Rapamycin (Serine/Threonine Kinase); FK506 Binding Protein 12-Rapamycin Associated Protein 2; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin Associated Protein FRAP2; FKBP-Rapamycin Associated Protein; Mechanistic Target Of Rapamycin; Rapamycin Target Protein 1; FRAP1; FRAP2
- Gene ID
- 2475
- UniProt ID
- P42345
- Long Name
- Mechanistic Target Of Rapamycin Kinase
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Related Signaling Pathways
BCR Signaling Pathway
ErbB Signaling Pathway
Insulin Signaling Pathway
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Related Diseases
Breast Cancer
Colorectal Cancer
EGFR Tyrosine Kinase Inhibitor Resistance
Gastric Cancer
Non-small Cell Lung Cancer
Hepatocellular Carcinoma
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
See other products for "MTOR"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0941MZ | Recombinant Mouse Anti-Human MTOR Antibody (clone 64F22) | ICC, IF | Mouse antibody |
| MRO-2048-CN | Rabbit Anti-MTOR Polyclonal Antibody (MRO-2048-CN) | IF, IHC, WB | Rabbit IgG |
| VS3-WK1564 | Rabbit Anti-MTOR Polyclonal Antibody | WB, ELISA | Rabbit IgG |
| VS3-XY3437 | Rabbit Anti-MTOR Recombinant Antibody (clone R07-8H4) | WB, IHC-P, ICC, IF, IP, FC | Rabbit IgG |
| VS3-WK2060 | Rabbit Anti-MTOR (pS2448) Recombinant Antibody (clone R13-9D-7) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2318 | Hi-Affi™ Recombinant Rabbit Anti-MTOR Monoclonal Antibody (DS2318AB) | ICC, IF, IHC-P, WB | IgG |
| MOR-4652 | Hi-Affi™ Recombinant Rabbit Anti-MTOR Monoclonal Antibody (TH165DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1390 | Anti-MTOR Immunohistochemistry Kit | IHC | |
| VS-0525-XY4569 | Anti-Mouse MTOR Immunohistochemistry Kit | IHC | |
| VS-0525-XY4568 | Anti-Human MTOR Immunohistochemistry Kit | IHC |
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