Rabbit Anti-NFKB2 Recombinant Antibody (VS13-YC798)
CAT#: VS13-YC798
The Rabbit Anti-NFKB2 Recombinant Antibody is specific for human, mouse and rat NFKB2. NFKB2 is part of the NF-kappa-B complex, activating inflammation and immune function genes. Depending on its dimerization partner, it acts as an activator or repressor and processes from p100 to p52. Chromosomal alterations are noted in B cell lymphomas, potentially forming fusion proteins, with multiple splice variants present. The anti-NFKB2 antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong positivity in enocrine and exocrine cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/ihc_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22098/if_selected.jpg
Normal Tissue
Figure 4 Appendix
High expression in appendix
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_1_2_val_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_7_5.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8422/23094_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HDLM-2, Karpas-707)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000077150-NFKB2
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Specifications
- Immunogen
- Recombinant human NFkB p100.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat NFKB2
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- Nuclear Factor Kappa B Subunit 2; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 (P49/P100); Lymphocyte Translocation Chromosome 10 Protein; DNA-Binding Factor KBF2; Oncogene Lyt-10; H2TF1; LYT10; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2; Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells 2; Nuclear Factor NF-Kappa-B P100 Subunit; Nuclear Factor NF-Kappa-B P52 Subunit;
- Gene ID
- 4791
- UniProt ID
- Q00653
- Long Name
- Nuclear Factor Kappa B Subunit 2
- Function
- Signal transduction
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Related Signaling Pathways
BCR Signaling Pathway
cAMP Signaling Pathway
NLR Signaling Pathway
TCR Signaling Pathway
TLR Signaling Pathway
TNF Signaling Pathway
Related Diseases
Maturity Onset Diabetes of the Young
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "NFKB2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2434 | Hi-Affi™ Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (DS2434AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1089-CN | Recombinant Rabbit Anti-NFKB2 Monoclonal Antibody (JM82-03) | WB, IF, IHC, IP | Rabbit IgG |
| FAMAB-1228CQ | Mouse Anti-NFKB2 Recombinant Antibody (clone SAIC-26C-15) | Immuno-MRM, ELISA | Mouse IgG1 |
| VS3-FY1034 | Recombinant Rabbit Anti-NFKB2 Antibody (clone R01-8B2) | WB, ICC, IF, IP | Rabbit IgG |
| VS7-0425-WR477 | Mouse Anti-NFKB2 Recombinant Antibody (clone 5E6A12) | WB, ELISA | Mouse IgG1 |
| VS7-0425-WR478 | Mouse Anti-NFKB2 Recombinant Antibody (clone 2F10A7) | FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1495 | Anti-NFKB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4819 | Anti-Mouse NFKB2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4818 | Anti-Human NFKB2 Immunohistochemistry Kit | IHC |
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