Mouse Anti-NHP ENG Recombinant Antibody (clone ENG/1621)

Name: Mouse Anti-NHP ENG Recombinant Antibody (clone ENG/1621)
SKU: VS-1024-XY186
Rating: 5 (1 reviews)

CAT#: VS-1024-XY186

Tailored for the detection of ENG, this mouse monoclonal antibody provides reactivity with human, non-human primate, pig, and rat samples. With a recombinant extracellular fragment of human ENG protein as the immunogen, it ensures accurate targeting. Ideal for immunofluorescence (IF) and flow cytometry (FC), this antibody delivers dependable results for exploring ENG expression and distribution across diverse biological systems.

Gene Expression

Subcellular Location Figure 1 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1823_F9_38_selected.jpg

Subcellular Location Figure 2 IHC staining of human placenta

Immunohistochemical staining of human placenta shows moderate membranous positivity in trophoblastic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/11862/158146_A_2_7_selected.jpg

Subcellular Location Figure 3 IF staining of human cell line BJ

Immunofluorescent staining of human cell line BJ shows localization to nuclear bodies & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1777_G1_13_cr59632eeaa8e82_blue_red_green.jpg

Subcellular Location Figure 4 IF staining of human cell line SiHa

Immunofluorescent staining of human cell line SiHa shows localization to nuclear bodies & plasma membrane.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/1759_C2_13_cr594b6f97edc10_blue_red_green.jpg

Normal Tissue Figure 5 Kidney

Cells in glomeruli
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/67440/156475_A_9_5.jpg

RNA Expression Figure 6 RNA cell line category: Cell line enhanced (fHDF/TERT166, HUVEC TERT2, TIME)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000106991-ENG

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Specifications

Immunogen
Recombinant extracellular fragment of human ENG protein

Host Species
Mouse

Type
Mouse IgG1, kappa

Specificity
ENG

Species Reactivity
Human, Non-human primate, Pig, Rat

Clone
ENG/1621

Applications
IF, FC

Product Property

Purification
Protein A purified

Purity
>95%, determined by SDS-PAGE

Format
Liquid

Buffer
PBS, pH 7.4, with 0.05% BSA

Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Applications

Application Notes
IF: 2-4 µg/mL, FC: 1-2 µg/10⁶ cells

Target

Alternative Names
END; HHT1; ORW1

Gene ID
2022

UniProt ID
P17813

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS-1024-XY186.pdf

MSDS

MSDS - VS-1024-XY186.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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Protocol & Troubleshooting

Immunofluorescence (IF)

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Recommended Dilution Buffer

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