SmartAb™ Recombinant Anti-NTRK2 pH-dependent Antibody (Clone C20)
CAT#: VS-0825-YC274
The anti-NTRK2 pH-dependent antibody (clone C20) is a recombinant monoclonal antibody that exhibits pH-dependent antigen binding to human, cynomolgus, and mouse Tropomyosin receptor kinase B (TrkB). By introducing histidine mutations that act as a pH-switch, the antibody demonstrates a preferential affinity for NTRK2 under acidic conditions compared to physiological pH. Using a yeast surface display system with pH-dependent screening pressure, this mAb is identified which shows a significant increase in dissociation from NTRK2 at pH 6.0. This pH-sensitive antibody retains its binding affinity on tumor cells while exhibiting negligible binding to the receptor on normal cells at physiological pH. NTRK2, also known as TrkB, is a receptor tyrosine kinase that binds to Brain-Derived Neurotrophic Factor (BDNF). It plays a crucial role in neuronal survival and synaptic plasticity, and its dysregulation is linked to mood disorders and certain cancers.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7637/202_C1_1_selected.jpg
Normal Tissue
Figure 2 High expression in cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10346/162989_B_7_5.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10346/162989_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10346/162989_A_6_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (AF22, HaCaT, RPTEC TERT1, SH-SY5Y, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000148053-NTRK2
❮
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Specifications
- Immunogen
- Recombinant TrkB protein.
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human, Cynomolgus and Mouse Tropomyosin receptor kinase B (TrkB)
- Species Reactivity
- Human, Cynomolgus, Mouse
- Clone
- C20
- Applications
- ELISA
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE and SEC-HPLC
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- For short-term storage, keep it at 4°C for up to two weeks. For long-term storage, divide the solution into aliquots and freeze them at -20°C or below.
Target
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Related Diseases
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone C20"
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-180CQ | Anti-Human NTRK2 Recombinant Antibody (C20) | FuncS | Human antibody |
| TAB-180CQ-S(P) | Anti-Human NTRK2 Recombinant Antibody scFv Fragment (C20) | ELISA, FC, WB | Human antibody |
| TAB-180CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (C20) | ELISA, FC, WB | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-177CQ-S(P) | Anti-Human NTRK2 Recombinant Antibody scFv Fragment (C2) | ELISA, FC | Humanized antibody |
| TAB-178CQ-S(P) | Anti-Human NTRK2 Recombinant Antibody scFv Fragment (A2) | ELISA, FC | Humanized antibody |
| TAB-177CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (C2) | ELISA, FC | Humanized antibody |
| TAB-178CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (A2) | ELISA, FC | Humanized antibody |
| TAB-179CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (RN1026A) | ELISA, FC | Humanized antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-171CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (18H6) | ELISA, FC | |
| TAB-173CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (6B72C5) | ELISA, WB, IP | |
| TAB-174CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (5G5D2B5) | ELISA, WB, IP | |
| TAB-175CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (17D11) | ELISA, FC, WB | |
| TAB-176CQ-F(E) | Anti-Human NTRK2 Recombinant Antibody Fab Fragment (29D7) | ELISA, FC, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0842MZ | Recombinant Mouse Anti-Human NTRK2 Antibody (clone 3D12) | WB | Mouse antibody |
| HPAB-N0197-YC | Mouse Anti-NTRK2 Recombinant Antibody (HPAB-N0197-YC) | FuncS, WB | Mouse IgG |
| HPAB-N0198-YC | Mouse Anti-NTRK2 Recombinant Antibody (HPAB-N0198-YC) | FuncS | Mouse IgG |
| VS3-WK1011 | Rabbit Anti-NTRK2 Recombinant Antibody (VS3-WK1011) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| VS3-FY2825 | Mouse Anti-NTRK2 Recombinant Antibody (VS3-FY2825) | WB, IHC, FC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2509 | Hi-Affi™ Recombinant Rabbit Anti-NTRK2 Monoclonal Antibody (DS2509AB) | WB, IHC, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NS-095CN | Human Anti-NTRK2 Recombinant Antibody (NS-095CN) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0662-CN-F(E) | Human Anti-NTRK2 Recombinant Antibody; Fab Fragment (HPAB-0662-CN-F(E)) | ELISA, Neut | Human Fab |
| HPAB-0663-CN-F(E) | Human Anti-NTRK2 Recombinant Antibody; Fab Fragment (HPAB-0663-CN-F(E)) | ELISA, Neut | Human Fab |
| HPAB-0664-CN-F(E) | Human Anti-NTRK2 Recombinant Antibody; Fab Fragment (HPAB-0664-CN-F(E)) | ELISA, Neut | Human Fab |
| HPAB-0665-CN-F(E) | Human Anti-NTRK2 Recombinant Antibody; Fab Fragment (HPAB-0665-CN-F(E)) | ELISA, Neut | Human Fab |
| HPAB-AP761-YC-F(E) | Human Anti-NTRK2 Recombinant Antibody (clone 4A6); Fab Fragment | Agonist, FuncS | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-J0197-YC-S(P) | Human Anti-NTRK2 Recombinant Antibody; scFv Fragment (HPAB-J0197-YC-S(P)) | ELISA | Human scFv |
| HPAB-J0198-YC-S(P) | Human Anti-NTRK2 Recombinant Antibody; scFv Fragment (HPAB-J0198-YC-S(P)) | ELISA | Human scFv |
| HPAB-J0199-YC-S(P) | Human Anti-NTRK2 Recombinant Antibody; scFv Fragment (HPAB-J0199-YC-S(P)) | ELISA | Human scFv |
| HPAB-J0200-YC-S(P) | Human Anti-NTRK2 Recombinant Antibody; scFv Fragment (HPAB-J0200-YC-S(P)) | ELISA | Human scFv |
| HPAB-J0201-YC-S(P) | Human Anti-NTRK2 Recombinant Antibody; scFv Fragment (HPAB-J0201-YC-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY99 | Human Anti-NTRK2 (clone 7-F1) scFv-Fc Chimera | IA | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC548 | Recombinant Anti-NTRK2 Vesicular Antibody, EV Displayed (VS-0425-YC548) | ELISA, FC, Neut, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5019 | Anti-NTRK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5020 | Anti-Mouse NTRK2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5021 | Anti-Rat NTRK2 Immunohistochemistry Kit | IHC |
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