Rabbit Anti-PCNA Polyclonal Antibody
CAT#: MRO-0211YJ
This product is a polyclonal rabbit antibody that recognizes PCNA.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
Normal Tissue
Figure 2 Colon
Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
Normal Tissue
Figure 3 Testis
Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
Normal Tissue
Figure 4 Bone marrow
Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
RNA Expression
Figure 6 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
❮
❯
Specifications
- Immunogen
- A synthesized peptide derived from human PCNA.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PCNA
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC, IF, ICC, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, pH 7.4, 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C for short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:3000
IHC: 1:50-1:200
IF/ICC: 1:100
ELISA: 1:20000-1:40000
Target
- Alternative Names
- ATLD2; cb16; Cyclin; DNA polymerase delta auxiliary protein; etID36690. 10; fa28e03; fb36g03; HGCN8729; MGC8367; Mutagen-sensitive 209 protein; OTTHUMP00000030189; OTTHUMP00000030190; PCNA; Pcna/cyclin; PCNA_HUMAN; PCNAR; Polymerase delta accessory protein; Proliferating cell nuclear antigen; wu:fa28e03; wu:fb36g03
- Gene ID
- 5111
- UniProt ID
- P12004
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "PCNA"
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|---|---|---|---|
| MOR-2616 | Hi-Affi™ Recombinant Rabbit Anti-PCNA Monoclonal Antibody (DS2616AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0205-YC-S(P) | Mouse Anti-PCNA Recombinant Antibody (clone 12D10); scFv Fragment | ELISA, WB, IHC | Mouse scFv |
| HPAB-N0376-YC-S(P) | Mouse Anti-PCNA Recombinant Antibody; scFv Fragment (HPAB-N0376-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0205-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody (clone 12D10); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
| HPAB-N0376-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody; Fab Fragment (HPAB-N0376-YC-F(E)) | ELISA, FC, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-0219YJ | Mouse Anti-PCNA Recombinant Antibody (clone CBS169) | WB, IHC, ELISA, FCM | Mouse IgG |
| MOB-0173F | Mouse Anti-PCNA Recombinant Antibody (clone 12D10), AbFluor™ 350 | WB, IF, IHC-P | Mouse IgG |
| MOB-0174F | Mouse Anti-PCNA Recombinant Antibody (clone 12D10), AbFluor™ 405 | WB, IF, IHC-P | Mouse IgG |
| MOB-0175F | Mouse Anti-PCNA Recombinant Antibody (clone 12D10), AbFluor™ 488 | WB, IF, IHC-P | Mouse IgG |
| MOB-0176F | Mouse Anti-PCNA Recombinant Antibody (clone 12D10), AbFluor™ 555 | WB, IF, IHC-P | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC76 | AbPlus™ Anti-PCNA Magnetic Beads (VS-0724-YC76) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY370 | Mouse Anti-NHP PCNA Recombinant Antibody (clone PC10) | WB, IHC, ICC, IP | Mouse IgG2a |
| CAT | Product Name | Application | Type |
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| VS13-YC876 | CytoStream™ Rabbit Anti-PCNA Recombinant Antibody (VS13-YC876) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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