Rabbit Anti-PIK3CA Recombinant Antibody (VS13-YC901)
CAT#: VS13-YC901
The Rabbit Anti-PIK3CA Recombinant Antibody is specific for human, mouse and rat PIK3CA. PIK3CA encodes a catalytic subunit of phosphatidylinositol 3-kinase, using ATP for phosphorylation. It's implicated in cervical and other cancers, with a pseudogene on chromosome 22. The anti-PIK3CA antibody can be used for Western blot (1/500-1/1000), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/68_B8_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human appendix
Immunohistochemical staining of human appendix shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17804/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24769_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9985/24713_A_9_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121879-PIK3CA
❮
❯
Specifications
- Immunogen
- Recombinant human PI 3 Kinase catalytic subunit alpha.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PIK3CA
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IP
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase Catalytic Subunit Alpha; Phosphoinositide-3-Kinase, Catalytic, Alpha Polypeptide; Serine/Threonine Protein Kinase PIK3CA; PtdIns-3-Kinase Subunit P110-Alpha; PI3K-Alpha; Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase Catalytic Subunit, Alpha Isoform; Phosphatidylinositol 4, 5-Bisphosphate 3-Kinase Catalytic Subunit Alpha Isoform; Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol 4, 5-Bisphosphate 3-Kinase 110 KDa Catalytic Subunit Alpha; Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase, Catalytic Subunit Alpha; Phosphatidylinositol 3-Kinase, Catalytic, Alpha Polypeptide; Phosphatidylinositol 3-Kinase, Catalytic, 110-KD, Alpha; Phosphoinositide-3-Kinase Catalytic Alpha Polypeptide; PI3-Kinase P110 Subunit Alpha; PtdIns-3-Kinase Subunit Alpha;
- Gene ID
- 5290
- UniProt ID
- P42336
- Long Name
- Phosphatidylinositol-4, 5-Bisphosphate 3-Kinase Catalytic Subunit Alpha
- Sequence Similarities
- Belongs to the PI3/PI4-kinase family.
- Cellular Localization
- Cytoplasm; Cytosol; Intercalated disc; Perinuclear region of cytoplasm; Plasma membrane
- Protein Refseq
- NP_006209.2
- Function
- Phosphoinositide-3-kinase (PI3K) phosphorylates phosphatidylinositol (PI) and its phosphorylated derivatives at position 3 of the inositol ring to produce 3-phosphoinositides (PubMed:15135396, PubMed:23936502, PubMed:28676499).
Uses ATP and PtdIns(4, 5)P2 (phosphatidylinositol 4, 5-bisphosphate) to generate phosphatidylinositol 3, 4, 5-trisphosphate (PIP3) (PubMed:15135396, PubMed:28676499).
PIP3 plays a key role by recruiting PH domain-containing proteins to the membrane, including AKT1 and PDPK1, activating signaling cascades involved in cell growth, survival, proliferation, motility and morphology. Participates in cellular signaling in response to various growth factors. Involved in the activation of AKT1 upon stimulation by receptor tyrosine kinases ligands such as EGF, insulin, IGF1, VEGFA and PDGF. Involved in signaling via insulin-receptor substrate (IRS) proteins. Essential in endothelial cell migration during vascular development through VEGFA signaling, possibly by regulating RhoA activity. Required for lymphatic vasculature development, possibly by binding to RAS and by activation by EGF and FGF2, but not by PDGF. Regulates invadopodia formation through the PDPK1-AKT1 pathway. Participates in cardiomyogenesis in embryonic stem cells through a AKT1 pathway. Participates in vasculogenesis in embryonic stem cells through PDK1 and protein kinase C pathway. In addition to its lipid kinase activity, it displays a serine-protein kinase activity that results in the autophosphorylation of the p85alpha regulatory subunit as well as phosphorylation of other proteins such as 4EBP1, H-Ras, the IL-3 beta c receptor and possibly others (PubMed:23936502, PubMed:28676499).
Plays a role in the positive regulation of phagocytosis and pinocytosis (By similarity).
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS13-YC901. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PIK3CA"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| AGTO-G056E | Anti-PIK3CA immunotoxin (scFv)-PE | Cytotoxicity assay, Function study |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2707 | Hi-Affi™ Recombinant Rabbit Anti-PIK3CA Monoclonal Antibody (DS2707AB) | ELISA, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0511U | Rabbit Anti-PIK3CA Recombinant Antibody (clone 10E5) | ELISA, WB, IF | Rabbit IgG |
| VS3-QX871 | Mouse Anti-PIK3CA Recombinant Antibody (clone 4F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ951 | Mouse Anti-PIK3CA Recombinant Antibody (clone ABT-PI3.H1047R) | WB, IF, IHC-P | Mouse IgG3, κ |
| VS3-CJ952 | Mouse Anti-PIK3CA Recombinant Antibody (clone PT0828) | WB, IHC-P | Mouse IgG1, κ |
| VS7-0425-WR612 | Mouse Anti-PIK3CA Monoclonal Antibody (clone 7H9B3 ) | IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1650 | Anti-PIK3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5405 | Anti-Mouse PIK3CA Immunohistochemistry Kit | IHC | |
| VS-0525-XY5404 | Anti-Human PIK3CA Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, IF, IP, Neut, FuncS, ELISA, FC
-2.png)
Application: ELISA, WB, BLI, SPR
Application: Neut, ELISA, FuncS
Application: FC, ADCC, CDC, Inhib
-2.png)
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
