Mouse Anti-PLCG2 Recombinant Antibody (clone 1E10C11) (CAT#: VS7-0425-WR629)
This product is a mouse IgG1 antibody that binds to human PLCG2. The antibody 1E10C11 recognizes specific amino acid domain of human PLCG2 (AA: 826-985). It can be used for IHC assay.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/235_H5_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/235_H5_1_selected.jpg
Figure 2 IHC staining of human appendix
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/ihc_selected.jpg
(Immunohistochemical staining of human appendix shows strong cytoplasmic positivity in lymphoid tissue.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/234_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/234_H5_1_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/535_H5_1_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/20099/535_H5_1_red_green.jpg
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18941_B_9_5.jpg
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18941_B_9_5.jpg
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_3.jpg
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_3.jpg
Figure 7 Kidney
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_5.jpg
(Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_5.jpg
Figure 8 Testis
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_6_6.jpg
(Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_6_6.jpg
Figure 9 Lymph node
(erminal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_8.jpg
(erminal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_8_8.jpg
Figure 10 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_5_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/4280/18882_A_5_8.jpg
Figure 11 RNA cell line category: Cell line enhanced (Daudi, HMC-1, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
Figure 11 RNA cell line category: Cell line enhanced (Daudi, HMC-1, REH, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000197943-PLCG2
Specifications
- Immunogen
- Recombinant human PLCG2 (AA: 826-985) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PLCG2
- Species Reactivity
- Human
- Clone
- 1E10C11
- Molecule Class
- Enzyme Phospholipase C Gamma 4
- Applications
- IHC
- Conjugate
- Unconjugated
- Related Disease
- Familial cold autoinflammatory syndrome 3 (FCAS3); Autoinflammation, antibody deficiency, and immune dysregulation (APLAID)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- FCAS3; APLAID; PLC-IV; PLC-gamma-2
- Gene ID
- 5336
- UniProt ID
- P16885
- Long Name
- Phospholipase C Gamma 2
- Cellular Localization
- Membrane
- Post Translation Modifications
- Phosphoprotein
- Protein Refseq
- NP_001412678.1
- Function
- Hydrolase; Transducer
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
BCR Signaling Pathway
cAMP Signaling Pathway
ErbB Signaling Pathway
FcεR1 Signaling Pathway
TCR Signaling Pathway
Non-small Cell Lung Cancer
Morphine Addiction
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1084z | Mouse Anti-PLCG2 Recombinant Antibody (clone 19A11) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| VS3-WK62 | Mouse Anti-PLCG2 Recombinant Antibody (clone E8-D9) | WB, ELISA | Mouse IgG1 |
| VS3-WK781 | Mouse Anti-PLCG2 Recombinant Antibody (clone Rac1) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-XY1270 | Mouse Anti-PLCG2 Recombinant Antibody (clone 1A7B8) | ELISA, WB, ICC, FC | Mouse IgG1 |
| VS3-FY2130 | Rabbit Anti-PLCG2 Recombinant Antibody (clone R42-7F-8) | WB, IHC-P, IP, FC | Rabbit IgG |
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