Afuco™ Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (CAT#: AFC-TAB-009)
This product is an ADCC enhanced antibody produced by our Afuco™ platform. Recombinant monoclonal antibody to Human RSV. Palivizumab is a monoclonal antibody produced by recombinant DNA technology. It is used in the prevention of respiratory syncytial virus (RSV) infections. It is recommended for infants that are high-risk because of prematurity or other medical problems such as congenital heart disease. Palivizumab is a humanized monoclonal antibody (IgG) directed against an epitope in the A antigenic site of the F protein of RSV.
Specific Inquiry
SDS-PAGE
Figure 1 Afuco Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (AFC-TAB-009) in SDS-PAGE
SDS-PAGE analysis of AFC-TAB-009 in reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result, different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
The purity of AFC-TAB-009 was greater than 95% as determined by SDS-PAGE.
SEC-HPLC
Figure 2 Afuco Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (AFC-TAB-009) in HPLC
The purity of AFC-TAB-009 was greater than 94% as determined by SEC-HPLC
Lectin WB
Figure 3 Afuco Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (AFC-TAB-009) in Lectin WB
Western blot analysis of AFC-TAB-009 was performed and fucose was detected by AOL-Biotin and SA-HRP. The result was negative, which indicates that there is almost no fucose produced.
Lane 1: Reducing AFC-TAB-009 (2 μg)
Lane 2: Reducing positive control antibody (2 μg)
Lectin ELISA
Figure 4 Afuco Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (AFC-TAB-009) in Lectin ELISA
Lectin ELISA analysis of AFC-TAB-009 was conducted and fucose was detected using AOL-Biotin and SA-HRP
The result indicates that there is almost no fucose produced.
WB
Figure 5 Afuco Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (AFC-TAB-009) in WB
Western blot analysis of AFC-TAB-009 was performed by loading RSV F.
AFC-TAB-009 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-anti-human IgG
Lane 1: Reducing Antigen (0.1 μg)
Lane 2: Reducing Antigen (0.3 μg)
Lane 3: Reducing Antigen (0.6 μg)
ELISA
Figure 6 Afuco Anti-RSV F ADCC Recombinant Antibody (Palivizumab), ADCC Enhanced (AFC-TAB-009) in ELISA
ELISA analysis of AFC-TAB-009 was performed by coating with RSV F.
The secondary antibody: HRP-anti-human IgG
Specifications
- Immunogen
- The details of the immunogen for this antibody are not available.
- Host Species
- Human
- Derivation
- Humanized (from mouse)
- Type
- ADCC enhanced antibody
- Specificity
- Tested positive against native RSV antigen
- Species Reactivity
- RSV
- Clone
- Palivizumab
- Applications
- IF, IP, Neut, FuncS, ELISA, FC
- Conjugate
- Unconjugated
- Trade name
- Synagis
- Generic Name
- Palivizumab
- Related Disease
- Respiratory Syncytial Virus (RSV) disease
Product Property
- Purity
- >95% by SDS-PAGE
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- Palivizumab; synagis; 188039-54-5; MEDI-493; humanized MAb1129; DB00110RSV; F; Fusion protein (F); NP_044596.1; UniProt ID: O09720; O09720; Entrez: 1494475; Entrez Gene: 1494475; Gene ID: 1494475; Palivizumab; Synagis; Palivizumab
Related Resources
Please refer to Palivizumab Overview to learn more about the mechanism of action, clinical projects, and approved drugs of Palivizumab.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Palivizumab"
Anti-RSV Recombinant Antibody (Palivizumab)Recombinant monoclonal antibody to Human RSV. Palivizumab is a monoclonal antibody produced by recombinant DNA technology. It is used in the prevention of respiratory syncytial virus (RSV) infections. It is recommended for infants that are high-risk because of prematurity or other medical problems such as congenital heart disease. Palivizumab is a humanized monoclonal antibody (IgG) directed against an epitope in the A antigenic site of the F protein of RSV.
See other products for "RSV F"
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Please select product type
Humanized Antibody
Human Antibody
Fab Fragment Antibody
Mouse Antibody
Recombinant Antibody
scFv Fragment Antibody
ADCC Enhanced Antibody
MHC Tetramer for Virology
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-009 | Anti-RSV Recombinant Antibody (Palivizumab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| TAB-709 | Anti-RSV glycoprotein F Recombinant Antibody (Motavizumab) | IF, IP, Neut, FuncS, ELISA, FC, WB | IgG1 - kappa |
| MRO-1209LC | Anti-HRSV F Recombinant Antibody (MEDI-493) | ELISA, Neut, PK | Humanized antibody |
| MRO-1209LC-S(P) | Anti-HRSV F Recombinant Antibody scFv Fragment (MEDI-493) | ELISA | Humanized antibody |
| MRO-1209LC-F(E) | Anti-HRSV F Recombinant Antibody Fab Fragment (MEDI-493) | ELISA | Humanized antibody |
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Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# AFC-TAB-009, RRID: AB_3111281)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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