Human Anti-SIRPA Recombinant Antibody (clone 218) (CAT#: PABJ-0078)
This product is a Human antibody that recognizes Human SIRPA. 218 binds SIRPα at regions distal from the CD47 binding site.
Specific Inquiry
ELISA
Figure 1 Human Anti-SIRPA Antibody (PABJ-0078) in ELISA
ELISA analysis of PABJ-0078 was performed by coating with Recombinant Human SIRPA.
The secondary antibody: HRP-Anti-Human IgG (H+L)
WB
Figure 2 Human Anti-SIRPA Antibody (PABJ-0078) in WB
Western blot analysis of PABJ-0078 was performed by loading Recombinant Human SIRPA.
PABJ-0078 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Lane1: Reducing antigen (0.1 μg)
Lane2: Reducing antigen (0.3 μg)
Lane3: Reducing antigen (0.6 μg)
DB
Figure 3 Human Anti-SIRPA Antibody (PABJ-0078) in DB
Dot blot analysis of PABJ-0078 was performed by loading Recombinant Human SIRPA.
PABJ-0078 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Anti-Human IgG (H+L)
Specifications
- Host Species
- Human
- Type
- Human IgG
- Specificity
- Human SIRPA
- Species Reactivity
- Human, Monkey
- Clone
- 218
- Applications
- ELISA
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay.
Target
- Alternative Names
- SIRPA; Inhibitory Receptor SHPS-1; Signal-Regulatory Protein Alpha-3; CD172A; CD172 Antigen-Like Family Member A; P84; Sirp-Alpha-1; Signal-Regulatory Protein Alpha-1; Sirp-Alpha-3; MFR; SHPS1; CD172a Antigen; Signal-Regulatory Protein Alpha-2; Macrophage
- Gene ID
- 140885
- UniProt ID
- P78324
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1201-VHH | Recombinant Anti-Mouse Sirpa VHH Single Domain Antibody | IP, FC, ChiP, Neut | Llama VHH |
Customer Reviews and Q&As
Customer Review
Q&As
-
Working well in WB
Great performance overall. The antibody worked well in Western blotting, giving us clear bands with minimal background. My only minor issue was with the initial dilution optimization, but customer service was very helpful in guiding us. -
Good for immunoprecipitation experiments
The greatest SIRPA antibody we have yet to utilize. In our immunoprecipitation studies, it has been quite helpful in delivering clear and powerful pull-downs that have improved our comprehension of the SIRPA signaling pathway.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0078, RRID: AB_3111603)
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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