Mouse Anti-SIRT1 Monoclonal Antibody (VS7-0425-WR854)
CAT#: VS7-0425-WR854
This product is a mouse IgG antibody that binds to human SIRT1. The antibody can be used for WB, IHC-P, IF, ICC, FC, and ELISA assays. It cross reacts with monkey.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/43_F3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52351/826_A6_1_selected.jpg
Normal Tissue
Figure 3 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_A_4_6.jpg
Normal Tissue
Figure 4 Adrenal gland
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6295/18379_B_6_5.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000096717-SIRT1
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Specifications
- Immunogen
- Recombinant human SIRT1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SIRT1
- Species Reactivity
- Human, Monkey
- Molecule Class
- Class III Histone Deacetylase
- Applications
- WB, IHC-P, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry: 1:200 - 1:1000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Target
- Alternative Names
- SIRT1; Sir2-Like 1; SIR2-Like Protein 1; Regulatory Protein SIR2 Homolog 1; Sirtuin 1; NAD-Dependent Protein Deacetylase Sirtuin-1; Sirtuin Type 1; SIR2alpha; Sirtuin (Silent Mating Type Information Regulation 2 Homolog) 1 (S. Cerevisiae); HSIRT1; Sirtuin
- Gene ID
- 23411
- UniProt ID
- Q96EB6
- Long Name
- Sirtuin 1
- Cellular Localization
- Cytoplasm; Mitochondrion; Nucleus
- Post Translation Modifications
- Acetylation; Methylation; Phosphoprotein; S-nitrosylation; Ubl conjugation
- Protein Refseq
- NP_001135970.1
- Function
- Developmental protein; Transferase
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Related Diseases
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "SIRT1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1383CT | Recombinant Mouse anti-Human SIRT1 Monoclonal antibody (2G4) | ELISA, FC, ICC, IF, IHC-P, WB | |
| MRO-1393-CN | Recombinant Mouse Anti-SIRT1 Monoclonal Antibody (7-C5-B2) | WB, IF, IHC | Mouse IgG2b |
| MRO-1394-CN | Recombinant Rabbit Anti-SIRT1 Monoclonal Antibody (CBACN-504) | WB, IHC, IP | Rabbit IgG |
| VS3-QX1010 | Mouse Anti-SIRT1 Recombinant Antibody (clone 2G10-F4-D9) | WB, IP | Mouse IgG2b |
| VS3-QX1011 | Mouse Anti-SIRT1 Recombinant Antibody (clone 1089CT5.3.1) | WB | Mouse IgG3, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0777MZ | Chicken Anti-SIRT1 Polyclonal IgY | IHC, IF, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3267 | Hi-Affi™ Recombinant Rabbit Anti-SIRT1 Monoclonal Antibody (DS3267AB) | IP, WB | IgG |
| MOR-0043-FY | Rabbit Anti-SIRT1 Recombinant Antibody (clone AFY0014) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY235 | AbPlus™ Anti-SIRT1 Magnetic Beads (CBACN-504) | IP, Protein Purification | |
| VS-0724-YC526 | AbPlus™ Anti-SIRT1 Magnetic Beads (VS-0724-YC526) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY480 | Mouse Anti-NHP SIRT1 Recombinant Antibody (clone 1F3) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2070 | Anti-SIRT1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6601 | Anti-Human SIRT1 Immunohistochemistry Kit | IHC |
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