Mouse Anti-SMAD5 Recombinant Antibody (VS7-0425-WR882) (CAT#: VS7-0425-WR882)
This product is a mouse IgG antibody that binds to human SMAD5. The antibody can be used for WB, IF, ICC, and FC assays. It cross reacts with mouse, rat, bovine, chicken, pig, and sheep.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/if_selected.jpg
Figure 2 IHC staining of human uterus, post-menopause
(Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
(Immunohistochemical staining of human uterus, post-menopause shows strong membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/ihc_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_7_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_9_4.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_8_5.jpg
Figure 6 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75% Peritubular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_A_5_6.jpg
Figure 7 Endometrium
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/58931/132096_B_1_3.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000113658-SMAD5
Specifications
- Immunogen
- Recombinant human SMAD5 (C-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SMAD5
- Species Reactivity
- Human, Mouse, Rat, Bovine, Chicken, Pig, sheep
- Molecule Class
- Smad Family
- Applications
- WB, IF, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Immunofluorescence: 1:100 - 1:500
Flow Cytometry: 1:100 - 1:200
Target
- Alternative Names
- SMAD5; Mothers Against DPP Homolog 5; Mothers Against Decapentaplegic, Drosophila, Homolog Of, 5; SMA- And MAD-Related Protein 5; MADH5; HSmad5; SMAD, Mothers Against DPP Homolog 5; SMAD, Mothers Against DPP Homolog 5 (Drosophila); MAD, Mothers Against De
- Gene ID
- 4090
- UniProt ID
- Q99717
- Long Name
- SMAD Family Member 5
- Cellular Localization
- Cytoplasm; Mitochondrion; Nucleus
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001001419.1
- Function
- DNA-binding
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3217z | Mouse Anti-SMAD5 Recombinant Antibody (clone 42D5) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MOB-1798MZ | Recombinant Mouse Anti-Human SMAD5 Antibody | ICC/IF, WB | Mouse antibody |
| ZG-0568U | Rabbit Anti-Phospho-SMAD5 (S463+S465) Recombinant Antibody (clone 2G11) | ELISA, IHC | Rabbit IgG |
| VS3-QX1029 | Mouse Anti-SMAD5 Recombinant Antibody (clone 4B10-B10-B6) | WB, ICC, FC | Mouse IgG1 |
| VS3-WK1119 | Rabbit Anti-SMAD5 Recombinant Antibody (VS3-WK1119) | WB, ICC, IHC, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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