Rabbit Anti-SPP1 Recombinant Antibody (clone 10A1) (CAT#: ZG-0075U)
The purified Rabbit Anti-SPP1 Recombinant Antibody (clone 10A1) displayed high specificity against the SPP1 protein of human.
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WB
Figure 1 Rabbit Anti-SPP1 Antibody (ZG-0075U) in WB
Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate. All lanes: Osteopontin Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 34, 33, 34, 34 kDa
Observed band size: 36 kDa
IHC
Figure 2 Rabbit Anti-SPP1 Antibody (ZG-0075U) in IHC
IHC image of ZG-0075U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Osteopontin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPP1
- Species Reactivity
- Human
- Clone
- 10A1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Pediatric Systemic Lupus Erythematosus and Urolithiasis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP OPN
- Gene ID
- 6696
- UniProt ID
- P10451
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Mouse Antibodies
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ScFv-Fc Chimeras
IHC Kits & Antibodies
Biparatopic Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0843CL | Anti-Human SPP1 Recombinant Antibody (2K1) | sELISA | |
| TAB-0844CL | Anti-Human SPP1 Recombinant Antibody (4C1) | sELISA | |
| TAB-0849CL | Anti-Human SPP1 Recombinant Antibody (2G2) | WB | |
| TAB-0849CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (2G2) | ELISA, FC | |
| TAB-0850CL-S(P) | Anti-Human SPP1 Recombinant Antibody scFv Fragment (2H2) | ELISA, FC |
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For Research Use Only. Not For Clinical Use.
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