Rabbit Anti-SPP1 Recombinant Antibody (clone 10A1) (CAT#: ZG-0075U)
The purified Rabbit Anti-SPP1 Recombinant Antibody (clone 10A1) displayed high specificity against the SPP1 protein of human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-SPP1 Antibody (ZG-0075U) in WB
Positive WB detected in: 293T whole cell lysate, U-251 whole cell lysate, U-87 whole cell lysate. All lanes: Osteopontin Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 36, 34, 33, 34, 34 kDa
Observed band size: 36 kDa
IHC
Figure 2 Rabbit Anti-SPP1 Antibody (ZG-0075U) in IHC
IHC image of ZG-0075U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Osteopontin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPP1
- Species Reactivity
- Human
- Clone
- 10A1
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Pediatric Systemic Lupus Erythematosus and Urolithiasis
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Osteopontin; Bone sialoprotein 1; Nephropontin; Secreted phosphoprotein 1; SPP-1; Urinary stone protein; Uropontin; SPP1; BNSP OPN
- Gene ID
- 6696
- UniProt ID
- P10451
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
Mouse Antibody
Human Antibody
Chicken IgY Antibody
Rabbit Monoclonal Antibody
scFv Fragment Antibody
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1403z | Mouse Anti-SPP1 Recombinant Antibody (clone 23F4) | ELISA, ICC, IF, IHC, WB | Mouse IgG1 |
| HPAB-0068-YC | Human Anti-SPP1 Recombinant Antibody (clone h2K1) | FuncS | Humanized IgG |
| HPAB-0069-YC | Human Anti-SPP1 Recombinant Antibody (clone c2K1) | FuncS | Chimeric (mouse/human) IgG, κ |
| HPAB-0070-YC | Human Anti-SPP1 Recombinant Antibody (clone R2K1v1.7) | ELISA | Humanized IgG |
| HPAB-1363-FY | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12) | Inhib, ELISA, WB | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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