Human Anti-SPP1 Recombinant Antibody (clone hu1A12) (CAT#: FAMAB-1252CQ)

Figure 1 ELISA assays.

Serial log dilutions of c1A12, the humanized version (hu1A12VHa/hu1A12VLa) or the humanized version (hu1A12VHc/hu1A12VLf) were added to ELISA plates pre-coated with human OPN and incubated for 1 h. After washing, mAbs binding to OPN were detected with HRP-conjugated goat antimouse IgG.

Dai, J., Li, B., Shi, J., Peng, L., Zhang, D., Qian, W., ... & Zhao, J. (2010). A humanized anti-osteopontin antibody inhibits breast cancer growth and metastasis in vivo. Cancer immunology, immunotherapy, 59(3), 355-366.

Figure 2 The inhibition eVects of hu1A12 on MDA-MB-435S cell migration, adhesion, invasion and colony formation in soft agar.

Cell migration was inhibited by hu1A12. After wounding, MDA-MB-435S monolayer cells were cultured in medium containing OPN in the presence of 25 μg/ml of hu1A12 or control human IgG. Photographs were taken immediately or 18 h after wounding under a Carl Zeiss Axiovert 135 microscope (×40).

Dai, J., Li, B., Shi, J., Peng, L., Zhang, D., Qian, W., ... & Zhao, J. (2010). A humanized anti-osteopontin antibody inhibits breast cancer growth and metastasis in vivo. Cancer immunology, immunotherapy, 59(3), 355-366.

Figure 3 EVect of anti-OPN antibodies on serum deprivation-induced apoptosis in MDA-MB-435S cells.

Bcl-2 family proteins were analyzed by Western blot analysis. The MDA-MB-435S cells were cultured in the presence or absence of OPN (with or without anti-OPN antibodies) for 24 h. After cultivation, we assessed the expression of Bcl-2 and Bax in MDA-MB-435S cells using Western blot analysis.

Dai, J., Li, B., Shi, J., Peng, L., Zhang, D., Qian, W., ... & Zhao, J. (2010). A humanized anti-osteopontin antibody inhibits breast cancer growth and metastasis in vivo. Cancer immunology, immunotherapy, 59(3), 355-366.

Figure 4 DAPI staining of apoptotic cells.

Note the nuclear condensation and fragmentation in the serum deprivation group, which was indicative of apoptosis.

Dai, J., Li, B., Shi, J., Peng, L., Zhang, D., Qian, W., ... & Zhao, J. (2010). A humanized anti-osteopontin antibody inhibits breast cancer growth and metastasis in vivo. Cancer immunology, immunotherapy, 59(3), 355-366.

Figure 5 hu1A12 eVectively suppresses MDA-MB-435S tumor growth and spontaneous lung metastasis in nude mice.

Efficacy of hu1A12 in inhibiting primary breast cancer growth.

Dai, J., Li, B., Shi, J., Peng, L., Zhang, D., Qian, W., ... & Zhao, J. (2010). A humanized anti-osteopontin antibody inhibits breast cancer growth and metastasis in vivo. Cancer immunology, immunotherapy, 59(3), 355-366.