Rabbit Anti-SPP1 Recombinant Antibody (VS3-FY2881) (CAT#: VS3-FY2881)
The anti-SPP1 antibody is a rabbit recombinant monoclonal antibody that has been designed to bind the human SPP1 protein. It cross-reacts with rat, hamster species. This monoclonal antibody proves valuable in disease research related to Pediatric Systemic Lupus Erythematosus and Dentin Dysplasia. The antibody can be utilized for detection of target protein by Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RPTEC TERT1
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27540/1789_G12_1_cr59549927dd49e_selected.jpg
(Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27540/1789_G12_1_cr59549927dd49e_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/1763_H3_6_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/1763_H3_6_selected.jpg
Figure 3 Kidney
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_7_5_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_7_5_val_selected.jpg
Figure 4 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_8_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_8_3.jpg
Figure 5 Gallbladder
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_6_4.jpg
(Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_6_4.jpg
Figure 6 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_9_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27541/64396_A_9_8.jpg
Figure 7 RNA cell line category: Cell line enhanced (EFO-21, Hep G2, RPTEC TERT1)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118785-SPP1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118785-SPP1
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Hamster, Rat SPP1
- Species Reactivity
- Human, Hamster, Rat
- Applications
- Western Blot, Immunohistochemistry
- Conjugate
- Unconjugated, FITC
- Related Disease
- Pediatric Systemic Lupus Erythematosus and Dentin Dysplasia
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Secreted Phosphoprotein 1; Early T-Lymphocyte Activation 1; Urinary Stone Protein; Nephropontin; Osteopontin; Uropontin; BNSP; OPN; Secreted Phosphoprotein 1 (Osteopontin, Bone Sialoprotein I, Early T-Lymphocyte Activation 1)
- Gene ID
- 6696
- UniProt ID
- P10451
- Sequence Similarities
- Belongs to the osteopontin family.
- Cellular Localization
- Secreted
- Post Translation Modifications
- Forms covalent cross-links mediated by transglutaminase TGM2, between a glutamine and the epsilon-amino group of a lysine residue, forming homopolymers and heteropolymers, increasing its collagen binding properties. (P10451-OSTP_HUMAN)
Extensively phosphorylated by FAM20C in the extracellular medium at multiple sites within the S-x-E/pS motif (PubMed:15869464, 22582013, 26091039).
The phosphorylated form inhibits hydroxyapatite crystallization.
Dephosphorylation via a mechanism involving ALPL/TNAP promotes hydroxyapatite crystallization (By similarity).
(P10451-OSTP_HUMAN)
O-glycosylated.
Isoform 5 is GalNAc O-glycosylated at Thr-59 or Ser-62.
- Protein Refseq
- NP_001035147.1; NP_001035149.1; NP_001238758.1; NP_001238759.1
- Function
- Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IgG Antibody Products
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ScFv-Fc Chimera Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1403z | Mouse Anti-SPP1 Recombinant Antibody (clone 23F4) | ELISA, ICC, IF, IHC, WB | Mouse IgG1 |
| HPAB-1363-FY | Mouse Anti-SPP1 Recombinant Antibody (clone 1A12) | Inhib, ELISA, WB | Mouse IgG |
| HPAB-1079WJ | Human Anti-SPP1 Recombinant Antibody (clone MOR-6990) | ELISA | Human IgG |
| HPAB-1080WJ | Human Anti-SPP1 Recombinant Antibody (clone MOR-6991) | ELISA | Human IgG |
| ZG-0182U | Mouse Anti-SPP1 Recombinant Antibody (clone 1B2C2) | ELISA, WB, IHC | Mouse IgG2a |
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