CytoStream™ Rabbit Anti-STAT1 Recombinant Antibody (VS13-YC1117) (CAT#: VS13-YC1117)

• Immunogen
• Recombinant human STAT1 alpha, aa 1-50.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human STAT1

• Species Reactivity
• Human

• Applications
• WB, ICC, IF, IHC-P, IP, FC

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/1000-1/5000
  Immunocytochemistry (ICC): 1/100-1/500
  Immunofluorescence (IF): 1/100-1/500
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
  Flow cytometry (FC): 1/50-1/100

• Alternative Names
• STAT91, ISGF-3

• Gene ID
• 6772

• UniProt ID
• P42224

• Long Name
• Signal Transducer And Activator Of Transcription 1

• Sequence Similarities
• Belongs to the transcription factor STAT family.

• Cellular Localization
• Nucleus; Cytoplasm

• Protein Refseq
• NP_009330.1; NP_644671.1

• Function
• Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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