Rabbit Anti-SYK Polyclonal Antibody
CAT#: VS3-WK1561
This antibody is a rabbit polyclonal antibody against human SYK, which is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen (synthesized peptide derived from human SYK). It can detect endogenous levels of SYK. This product was tested for use in WB and ELISA.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Normal Tissue
Figure 8 Tonsil
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
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Specifications
- Immunogen
- Synthesized peptide derived from human SYK
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SYK
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Aggregation
- < 5%
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 0.5% BSA, 50% Glycerol
- Preservative
- 0.02% Sodium Azide
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot (1:500) and ELISA (1:10000-1/20000).
Target
- Alternative Names
- IMD82; p72-Syk
- Gene ID
- 6850
- UniProt ID
- P43405
- Long Name
- Spleen associated tyrosine kinase
- Sequence Similarities
- Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane
- Post Translation Modifications
- Ubiquitinated by CBLB after BCR activation; which promotes proteasomal degradation.
- Protein Refseq
- NP_001128524.1
- Function
- Non-receptor tyrosine kinase which mediates signal transduction downstream of a variety of transmembrane receptors including classical immunoreceptors like the B-cell receptor (BCR). Regulates several biological processes including innate and adaptive immunity, cell adhesion, osteoclast maturation, platelet activation and vascular development.
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Related Signaling Pathways
BCR Signaling Pathway
FcεR1 Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937 | Recombinant Anti-human SYK Antibody | WB, ELISA, IHC, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
| CAT | Product Name | Application | Type |
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| MOB-937-S(P) | Recombinant Anti-human SYK Antibody scFv Fragment | ELISA, WB, FC, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| MHH-937 | Recombinant Human Anti-human SYK Antibody | ELISA, WB, Dot, FuncS | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-937-F(E) | Recombinant Human Anti-human SYK Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-937-S(P) | Recombinant Human Anti-human SYK Antibody scFv Fragment | ELISA, WB, IP, FuncS | scFv |
| CAT | Product Name | Application | Type |
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| MOB-1977MZ | Recombinant Mouse Anti-Human SYK Antibody (clone 8H5) | WB | Mouse antibody |
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| MOR-3476 | Hi-Affi™ Recombinant Rabbit Anti-SYK Monoclonal Antibody (DS3476AB) | ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0225-YC | Rabbit Anti-SYK Recombinant Antibody (HPAB-0225-YC) | IHC, FuncS | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| HPAB-0225-YC-S(P) | Rabbit Anti-SYK Recombinant Antibody; scFv Fragment (HPAB-0225-YC-S(P)) | IHC, FuncS | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-123R | Mouse Anti-SYK Recombinant Antibody (ZG-123R) | WB, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
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| VS-0425-FY47 | Rabbit Anti-SYK (clone MIL81-1-8) scFv-Fc Chimera | IHC | Rabbit IgG, scFv-Fc |
| CAT | Product Name | Application | Type |
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| VS-0525-XY7041 | Anti-SYK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
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| VS-0525-XY7042 | Anti-Mouse SYK Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0825-YC318 | SmartAb™ Recombinant Anti-SYK pH-dependent Antibody (VS-0825-YC318) | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1025-YC61 | Anti-SYK Antibody Prodrug, Protease Activated (VS-1025-YC61) | ISZ, Cyt, FuncS |
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