Mouse Anti-SYK Recombinant Antibody (clone 2G11E6) (CAT#: VS7-0425-WR940)
This product is a mouse IgG1 antibody that binds to human SYK. The antibody 2G11E6 recognizes specific amino acid domain of human SYK (AA: 217-356). It can be used for ICC and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Figure 2 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Figure 3 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Figure 5 Kidney
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Figure 6 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Figure 8 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
Specifications
- Immunogen
- Recombinant human SYK (AA: 217-356) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SYK
- Species Reactivity
- Human
- Clone
- 2G11E6
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency 82 with systemic inflammation (IMD82)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunocytochemistry: 1:50 - 1:250
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SYK; spleen tyrosine kinase; p72-Syk; tyrosine-protein kinase SYK
- Gene ID
- 6850
- UniProt ID
- A0A024R273
- Long Name
- Spleen associated tyrosine kinase
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001128524.1
- Function
- Kinase; Transferase; Tyrosine-protein kinase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
BCR Signaling Pathway
FcεR1 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
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| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
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