Mouse Anti-SYK Recombinant Antibody (clone 3D1G10) (CAT#: VS7-0425-WR941)
This product is a mouse IgG1 antibody that binds to human SYK. The antibody 3D1G10 recognizes specific amino acid domain of human SYK (AA: 217-356). It can be used for WB and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to plasma membrane & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/1384/1472_A1_6_selected.jpg
Figure 2 Cerebral cortex
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
(Glial cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_B_9_5.jpg
Figure 3 Colon
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
(Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_8_3.jpg
Figure 4 Liver
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
(Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_9_4.jpg
Figure 5 Kidney
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
(Collecting ducts
Staining: Low
Intensity: Moderate
Quantity: <25%
Distal tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_5.jpg
Figure 6 Testis
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
(Sertoli cells
Staining: Medium
Intensity: Moderate
Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_6.jpg
Figure 7 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_7_8.jpg
Figure 8 Tonsil
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Squamous epithelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7773/18512_A_6_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (Daudi, HEL, HL-60, HMC-1, REH, THP-1, U-698, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165025-SYK/subcellular
Specifications
- Immunogen
- Recombinant human SYK (AA: 217-356) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SYK
- Species Reactivity
- Human
- Clone
- 3D1G10
- Molecule Class
- Non-Receptor Tyrosine Kinase
- Applications
- WB, FC
- Conjugate
- Unconjugated
- Related Disease
- Immunodeficiency 82 with systemic inflammation (IMD82)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- SYK; spleen tyrosine kinase; p72-Syk; tyrosine-protein kinase SYK
- Gene ID
- 6850
- UniProt ID
- A0A024R273
- Long Name
- Spleen associated tyrosine kinase
- Cellular Localization
- Cell membrane; Cytoplasm; Membrane
- Post Translation Modifications
- Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001128524.1
- Function
- Kinase; Transferase; Tyrosine-protein kinase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Related Resources
BCR Signaling Pathway
FcεR1 Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
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| MOB-937-F(E) | Recombinant Anti-human SYK Antibody Fab Fragment | IF, FACS, FuncS | Fab |
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| HPAB-0225-YC-F(E) | Rabbit Anti-SYK Recombinant Antibody; Fab Fragment (HPAB-0225-YC-F(E)) | IHC, FuncS | Rabbit Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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