Recombinant monoclonal antibody specially binds to VI of human TPO. This human anti-TPO monoclonal antibody B8 can be widely used in the treatment of Graves' disease.
Figure 1 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in ELISA
ELISA analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag). Then blocked with BSA and incubated with FAMAB-0014JF. A HRP-conjugated goat anti-human IgG (H+L) was used as the secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
Figure 2 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in WB
Western blot analysis of FAMAB-0014JF was performed by loading human TPO protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0014JF and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 4000). Chemiluminescent detection was performed.
Figure 3 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in DB
Dot Blot analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag).
FAMAB-0014JF incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 4000)
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-999 | Recombinant Anti-TPO Antibody | ELISA, WB, FuncS | IgG |
MOB-1174MZ | Recombinant Mouse Anti-Human TPO Antibody (clone NpBc58) | IHC-P | Mouse antibody |
FAMAB-0036JF | Human Anti-TPO Recombinant Antibody (clone TF3.5) | WB, ELISA, FC | Human IgG |
FAMAB-0042JF | Human Anti-TPO Recombinant Antibody (clone ICA5) | WB, ELISA, FuncS | Human IgG |
FAMAB-0364JF | Human Anti-TPO Recombinant Antibody (clone RI-34) | ELISA, FuncS | Human IgG |
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# FAMAB-0014JF, RRID: AB_3111302)
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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