Human Anti-TPO Recombinant Antibody (clone B8) (CAT#: FAMAB-0014JF)

Recombinant monoclonal antibody specially binds to VI of human TPO. This human anti-TPO monoclonal antibody B8 can be widely used in the treatment of Graves' disease.

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ELISA

Figure 1 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in ELISA

Figure 1 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in ELISA

ELISA analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag). Then blocked with BSA and incubated with FAMAB-0014JF. A HRP-conjugated goat anti-human IgG (H+L) was used as the secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

WB

Figure 2 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in WB

Figure 2 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in WB

Western blot analysis of FAMAB-0014JF was performed by loading human TPO protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0014JF and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 4000). Chemiluminescent detection was performed.

DB

Figure 3 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in DB

Figure 3 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in DB

Dot Blot analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag).
FAMAB-0014JF incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 4000)


Specifications

  • Host Species
  • Human
  • Derivation
  • Human phage display scFv library
  • Type
  • Human IgG
  • Specificity
  • Human TPO
  • Species Reactivity
  • Human
  • Clone
  • B8
  • Applications
  • WB, ELISA, IHC
  • Related Disease
  • Graves' disease

Product Property

  • Purity
  • >95% as determined by SDS-PAGE and HPLC analysis
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • No preservatives
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • The TPO antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry.

Target

  • Alternative Names
  • MSA; TPX; TDH2A
  • Function
  • Inhibitor

Related Resources

  • Related Diseases
  • Citations

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Customer Reviews and Q&As

Customer Review Q&As

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  • Great for TPO Studies
    The Anti-TPO Recombinant Antibody (clone B8) has been excellent for our TPO studies. Its specificity and sensitivity are top-notch, providing clear and reliable results. This antibody has significantly improved the accuracy of our experiments and saved us a lot of troubleshooting time.
  • Reliable in Multiple Assays
    We incorporated this antibody into our Western blot and immunoassays, and it has been a great asset. The specificity for TPO is precise, allowing for accurate detection and analysis. The reliable performance has made it an invaluable tool in our lab.
  • Consistent High-Quality Results
    This antibody has proven to be consistent and high-quality in our various assays. The reproducible results and strong affinity for TPO have significantly improved our experiments, making it a crucial part of our research toolkit. The consistent performance has greatly enhanced our data quality.

Cite This Product

To accurately reference this product in your publication, please use the following citation information:

(Creative Biolabs Cat# FAMAB-0014JF, RRID: AB_3111302)

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For Research Use Only. Not For Clinical Use.

For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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