Human Anti-TPO Recombinant Antibody (clone B8) (CAT#: FAMAB-0014JF)

Figure 1 Effect of a reducing agent on the pattern of the immunoreactive bands in Western blots.

Western blot of microsomal proteins incubated with graded amounts of dithiothreitol in the sample treatment buffer.

Portmann, L., Fitch, F. W., Havran, W., Hamada, N., Franklin, W. A., & DeGroot, L. J. (1988). Characterization of the thyroid microsomal antigen, and its relationship to thyroid peroxidase, using monoclonal antibodies. The Journal of clinical investigation, 81(4), 1217-1224.

Figure 2 Double immunoprecipitation of TPO enzymatic activity by the MAb anti-microsomal antigen.

Peroxidase activity was measured by the guaiacol method.

Portmann, L., Fitch, F. W., Havran, W., Hamada, N., Franklin, W. A., & DeGroot, L. J. (1988). Characterization of the thyroid microsomal antigen, and its relationship to thyroid peroxidase, using monoclonal antibodies. The Journal of clinical investigation, 81(4), 1217-1224.

Figure 3 Staining of thyroid colloid nodule with an MAb against microsomal antigen.

Representative results from one of two independent experiments are shown.

Portmann, L., Fitch, F. W., Havran, W., Hamada, N., Franklin, W. A., & DeGroot, L. J. (1988). Characterization of the thyroid microsomal antigen, and its relationship to thyroid peroxidase, using monoclonal antibodies. The Journal of clinical investigation, 81(4), 1217-1224.

Figure 1 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in ELISA

ELISA analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag). Then blocked with BSA and incubated with FAMAB-0014JF. A HRP-conjugated goat anti-human IgG (H+L) was used as the secondary antibody (1: 6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.

Figure 2 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in WB

Western blot analysis of FAMAB-0014JF was performed by loading human TPO protein (Lane 1 0.1 μg, Lane 2 0.3 μg, Lane 3 0.6 μg) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with FAMAB-0014JF and a HRP goat anti-human IgG (H+L) was used as the secondary antibody (1: 4000). Chemiluminescent detection was performed.

Figure 3 Anti-TPO Recombinant Antibody (FAMAB-0014JF) in DB

Dot Blot analysis of FAMAB-0014JF was performed by coating with human TPO protein (His tag).
FAMAB-0014JF incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-human IgG (H+L) secondary antibody (1: 4000)