Human Anti-TPO Recombinant Antibody (clone T5) (CAT#: FAMAB-0015JF)
Recombinant monoclonal antibody specially binds to VI/VIII of human TPO. This human anti-TPO antibody T5 can be widely used in the treatment of Graves' disease.
Specific Inquiry
WB
Figure 1 Western blot analyses of RNA and protein, respectively, from transfected cell line.
The proteins were electrotransferred to nitrocellulose membranes and then probed with a mouse MAb against the thyroid microsomal antigen.
Kaufman, K. D., Rapoport, B., Seto, P., Chazenbalk, G. D., & Magnusson, R. P. (1989). Generation of recombinant, enzymatically active human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto's thyroiditis. The Journal of clinical investigation, 84(2), 394-403.
FC
Figure 2 FACS analysis of CHO cells transfected with pHTPOECE.
Cells exposed to phycoerythrin (PE)-labeled second antibody alone, without prior exposure to human serum.
Kaufman, K. D., Rapoport, B., Seto, P., Chazenbalk, G. D., & Magnusson, R. P. (1989). Generation of recombinant, enzymatically active human thyroid peroxidase and its recognition by antibodies in the sera of patients with Hashimoto's thyroiditis. The Journal of clinical investigation, 84(2), 394-403.
IHC
Figure 3 Immunohistochemical staining for thyroid peroxidase (TPO) in nonmalignant and malignant thyroid lesions (indirect immunoperoxidase technique.
Strong immunostaining for TPO in Graves' disease.
Savin, S., Cvejić, D., Išić, T., Petrović, I., Paunović, I., Tatić, S., & Havelka, M. (2006). Thyroid peroxidase immunohistochemistry in differential diagnosis of thyroid tumors. Endocrine pathology, 17(1), 53-60.
ELISA
Figure 4 TPO antibodies measured by ELISA.
The data are expressed as absorbance at 450 nm (A450).
Guo, J., Pichurin, P., Nagayama, Y., Rapoport, B., & McLachlan, S. M. (2003). Insight into antibody responses induced by plasmid or adenoviral vectors encoding thyroid peroxidase, a major thyroid autoantigen. Clinical & Experimental Immunology, 132(3), 408-415.
Specifications
- Host Species
- Human
- Derivation
- Human phage display scFv library
- Type
- Human IgG
- Specificity
- Human TPO
- Species Reactivity
- Human
- Clone
- T5
- Applications
- WB, ELISA, IHC, FC
- Related Disease
- Graves' disease
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The TPO antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Flow Cytometry.
Target
Related Resources
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-999-S(P) | Recombinant Anti-TPO Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| MHH-999-S(P) | Recombinant Human Anti-TPO Antibody scFv Fragment | WB, FC, FuncS | scFv |
| PSBW-123 | Human Anti-TPO Recombinant Antibody (clone TR1.9); scFv Fragment | WB, ELISA | Human scFv |
| FAMAB-0042JF-S(P) | Human Anti-TPO Recombinant Antibody (clone ICA5); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| FAMAB-0364JF-S(P) | Human Anti-TPO Recombinant Antibody (clone RI-34); scFv Fragment | ELISA, FuncS | Human scFv |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2215CQ | Mouse Anti-TPO Recombinant Antibody (clone CBL296) | Neut, ELISA | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3658 | Rabbit Anti-TPO Recombinant Antibody (clone DS3658AB) | IHC-P, IP, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4548 | PE-DQB1*03:02/Human TPO (IDVWLGGLAENFLP) MHC Tetramer | FCM |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2017-FY-F(E) | Human Anti-TPO Recombinant Antibody (clone FY-019); Fab Fragment | ELISA | Human Fab |
| FAMAB-0013JF-F(E) | Human Anti-TPO Recombinant Antibody (clone A12); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| FAMAB-0014JF-F(E) | Human Anti-TPO Recombinant Antibody (clone B8); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| FAMAB-0015JF-F(E) | Human Anti-TPO Recombinant Antibody (clone T5); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| FAMAB-0036JF-F(E) | Human Anti-TPO Recombinant Antibody (clone TF3.5); Fab Fragment | WB, ELISA, FuncS | Human Fab |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0013JF | Human Anti-TPO Recombinant Antibody (clone A12) | WB, ELISA, IHC, Inhib | Human IgG |
| FAMAB-0014JF | Human Anti-TPO Recombinant Antibody (clone B8) | WB, ELISA, IHC | Human IgG |
| FAMAB-0036JF | Human Anti-TPO Recombinant Antibody (clone TF3.5) | WB, ELISA, FC | Human IgG |
| FAMAB-0042JF | Human Anti-TPO Recombinant Antibody (clone ICA5) | WB, ELISA, FuncS | Human IgG |
| FAMAB-1281CQ | Human Anti-TPO Recombinant Antibody (clone WR1.7) | ELISA, Inhib | Human IgE |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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