Human Anti-TPO Recombinant Antibody (clone TF3.5) (CAT#: FAMAB-0036JF)
Recombinant monoclonal antibody can specifically bind TPO outside the immunodominant region. This human anti-non-IDR antibody TF3.5 can be potentially used in the treatment of patients with TPO autoantibody-positive Graves.
Specific Inquiry
WB
Figure 1 WB of solubilized thyroid membrane fractions and immunoblotting of sera from patients with Hashi moto's thyroiditis.
Mag=microsomal antigen. Tg=thyroglobulin.
Bogner, U., Kotulla, P., Peters, H., & Schleusener, H. (1990). Thyroid peroxidase/microsomal antibodies are not identical with thyroid cytotoxic antibodies in autoimmune thyroiditis. European Journal of Endocrinology, 123(4), 431-437.
ELISA
Figure 2 Specific lysis and titres of microsomal antibodies determined by ELISA.
The data are presented in OD at 405 nm.
Bogner, U., Kotulla, P., Peters, H., & Schleusener, H. (1990). Thyroid peroxidase/microsomal antibodies are not identical with thyroid cytotoxic antibodies in autoimmune thyroiditis. European Journal of Endocrinology, 123(4), 431-437.
FC
Figure 3 Flow cytometric analysis of IgG class TSHR autoantibody binding to CHO cells expressing different numbers of TSHR on their surface.
TSHR wild-type (WT) cells are stably transfected with the 4-kilobase TSH cDNA.
Jaume, J. C., Kakinuma, A., Chazenbalk, G. D., Rapoport, B., & McLachlan, S. M. (1997). Thyrotropin receptor autoantibodies in serum are present at much lower levels than thyroid peroxidase autoantibodies: analysis by flow cytometry. The Journal of Clinical Endocrinology & Metabolism, 82(2), 500-507.
Specifications
- Host Species
- Human
- Derivation
- Human phage display Fab library
- Type
- Human IgG
- Specificity
- Human non-IDR TPO
- Species Reactivity
- Human
- Clone
- TF3.5
- Applications
- WB, ELISA, FC
- Related Disease
- TPO autoantibody-positive Graves
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The TPO antibody has been reported in applications of Western Blot, Enzyme-linked Immunosorbent Assay, Flow Cytometry.
Target
Related Resources
Autoimmune Thyroid Disease
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-999 | Recombinant Anti-TPO Antibody | ELISA, WB, FuncS | IgG |
| MHH-999 | Recombinant Human Anti-TPO Antibody | WB, ELISA, IHC, FuncS | IgG |
| PABW-123 | Human Anti-TPO Recombinant Antibody (clone TR1.9) | Inhib | Human IgG |
| MOB-1174MZ | Recombinant Mouse Anti-Human TPO Antibody (clone NpBc58) | IHC-P | Mouse antibody |
| HPAB-2017-FY | Human Anti-TPO Recombinant Antibody (clone FY-019) | ELISA | Human IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-999-F(E) | Recombinant Anti-TPO Antibody Fab Fragment | ELISA, Nert, FuncS | Fab |
| MHH-999-F(E) | Recombinant Human Anti-TPO Antibody Fab Fragment | IP, IF, FuncS | Fab |
| FAMAB-0013JF-F(E) | Human Anti-TPO Recombinant Antibody (clone A12); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| FAMAB-0014JF-F(E) | Human Anti-TPO Recombinant Antibody (clone B8); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| FAMAB-0015JF-F(E) | Human Anti-TPO Recombinant Antibody (clone T5); Fab Fragment | WB, ELISA, FuncS | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHH-999-S(P) | Recombinant Human Anti-TPO Antibody scFv Fragment | WB, FC, FuncS | scFv |
| PSBW-123 | Human Anti-TPO Recombinant Antibody (clone TR1.9); scFv Fragment | WB, ELISA | Human scFv |
| HPAB-2017-FY-S(P) | Human Anti-TPO Recombinant Antibody (clone FY-019); scFv Fragment | ELISA | Human scFv |
| FAMAB-0042JF-S(P) | Human Anti-TPO Recombinant Antibody (clone ICA5); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| FAMAB-0364JF-S(P) | Human Anti-TPO Recombinant Antibody (clone RI-34); scFv Fragment | ELISA, FuncS | Human scFv |
Neutralizing Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2215CQ | Mouse Anti-TPO Recombinant Antibody (clone CBL296) | Neut, ELISA | Mouse IgG2b |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3658 | Rabbit Anti-TPO Recombinant Antibody (clone DS3658AB) | IHC-P, IP, WB | Rabbit IgG |
MHC Tetramer for Cancer
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC4548 | PE-DQB1*03:02/Human TPO (IDVWLGGLAENFLP) MHC Tetramer | FCM |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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