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    Rabbit Anti-VIM Recombinant Antibody (VS3-CJ515) (CAT#: VS3-CJ515)

    This product is a rabbit antibody that recognizes human, mouse, and rat VIM.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line ASC TERT1
    Figure 1 IF staining of human cell line ASC TERT1
    (Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
    Figure 2 IF staining of human cell line U-2 OS
    Figure 2 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
    Figure 3 IF staining of human cell line U-251 MG
    Figure 3 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
    Normal Tissue
    Figure 4 Cerebral cortex
    Figure 4 Cerebral cortex
    (Endothelial cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    nuclear
    Glial cells
    Staining:

    Medium

    Intensity: Strong
    Quantity: <25%
    Location: Cytoplasmic/
    membranous

    Neuropil
    Staining:

    Medium

    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/
    membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
    Figure 5 Colon
    Figure 5 Colon
    (Endothelial cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%

    Fibroblasts
    Staining:

    High

    Intensity: Strong

    Mucosal lymphoid cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
    Figure 6 Kidney
    Figure 6 Kidney
    (Cells in glomeruli
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    Cells in tubules
    Staining:

    Medium

    Intensity: Strong
    Quantity: <25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
    Figure 7 Testis
    Figure 7 Testis
    (Leydig cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Pachytene spermatocytes
    Staining:

    High

    Intensity: Strong
    Quantity: 75%-25%
    Peritubular cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Preleptotene spermatocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%

    Sertoli cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Spermatogonia cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
    Figure 8 Breast
    Figure 8 Breast
    (Adipocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    nuclear

    Myoepithelial cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
    Figure 9 Lymph node
    Figure 9 Lymph node
    (Germinal center cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    Non-germinal center cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
    RNA Expression
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    (Cell lines ordered by descending RNA expression order.)
    * https://v21.proteinatlas.org/ENSG00000026025-VIM
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM

    Specifications

    • Immunogen
    • Recombinant protein
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human, Mouse, Rat VIM
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB, ICC, IF, IHC, IP, FC
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Protein A affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • 40% Glycerol, 1% BSA, TBS, pH7.4.
    • Preservative
    • 0.05% Sodium Azide
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.

    Target

    • Alternative Names
    • Vimentin
    • Sequence Similarities
    • Belongs to the intermediate filament family.
    • Cellular Localization
    • Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane, Nucleus
    • Post Translation Modifications
    • Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
      One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
      Phosphorylated by STK33 (PubMed:18811945).
      Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
      O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
      S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
    • Function
    • Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
      Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "VIM"

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    CAT Product Name Application Type
    TAB-353CQ Anti-Human VIM Recombinant Antibody (HoAKs-1) ELISA
    TAB-354CQ Anti-Human VIM Recombinant Antibody (2F6-1) IF
    TAB-353CQ-S(P) Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1) ELISA, WB
    TAB-354CQ-S(P) Anti-Human VIM Recombinant Antibody scFv Fragment (2F6-1) ELISA, WB
    TAB-355CQ-S(P) Anti-Human VIM Recombinant Antibody scFv Fragment (3F9-1) ELISA, WB

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