Rabbit Anti-VIM Recombinant Antibody (VS3-CJ515)
CAT#: VS3-CJ515
This product is a rabbit antibody that recognizes human, mouse, and rat VIM.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line ASC TERT1
Immunofluorescent staining of human cell line ASC TERT1 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/1774_A1_22_cr5950f6319513b_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/32_C3_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1762/34_C3_1_blue_red_green.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Glial cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/
membranous
Neuropil
Staining:
Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/
membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Fibroblasts
Staining:
High
Intensity: Strong
Mucosal lymphoid cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Cells in tubules
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining:
High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Peritubular cells
Staining:
High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:
High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:
High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_6_6.jpg
Normal Tissue
Figure 8 Breast
Adipocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
Myoepithelial cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1706_B_2_4.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Non-germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/80/1664_B_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (ASC TERT1, fHDF/TERT166)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000026025-VIM
❮
❯
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat VIM
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- Vimentin
- Gene ID
- 7431
- UniProt ID
- P08670
- Sequence Similarities
- Belongs to the intermediate filament family.
- Cellular Localization
- Cell membrane, Cytoplasm, Cytoskeleton, Intermediate filament, Membrane, Nucleus
- Post Translation Modifications
- Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity).
One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480).
Phosphorylated by STK33 (PubMed:18811945).
Phosphorylated on tyrosine residues by SRMS (PubMed:29496907).
O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status.
S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
- Protein Refseq
- NP_003371.2
- Function
- Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-CJ515. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "VIM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-353CQ | Anti-Human VIM Recombinant Antibody (HoAKs-1) | ELISA | |
| TAB-354CQ | Anti-Human VIM Recombinant Antibody (2F6-1) | IF | |
| TAB-353CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (HoAKs-1) | ELISA, WB | |
| TAB-354CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (2F6-1) | ELISA, WB | |
| TAB-355CQ-S(P) | Anti-Human VIM Recombinant Antibody scFv Fragment (3F9-1) | ELISA, WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0741MZ | Chicken Anti-VIM Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3838 | Rabbit Anti-VIM Recombinant Antibody (clone DS3838AB) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-F(E) | Human Anti-VIM Recombinant Antibody Fab Fragment (HPAB-2717LY-F(E)) | FC, WB, Block, ELISA | Humanized Fab |
| HPAB-0349-YJ-F(E) | Mouse Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0349-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse Fab |
| HPAB-0350-YJ-F(E) | Human Anti-VIM Recombinant Antibody; Fab Fragment (HPAB-0350-YJ-F(E)) | ELISA, WB, IP, ICC, IHC, FC, IF | Humanized Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-2717LY-S(P) | Human Anti-VIM Recombinant Antibody scFv Fragment (HPAB-2717LY-S(P)) | FC, WB, Block, ELISA | Humanized scfv |
| HPAB-0349-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0349-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| HPAB-0350-YJ-S(P) | Mouse Anti-VIM Recombinant Antibody; scFv Fragment (HPAB-0350-YJ-S(P)) | ELISA, WB, IP, ICC, IHC, FC, IF | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF038 | DRB1*04:01/Human cit vimentin (GVYAT[cit]SSAV[cit]L[cit]SSVPGVR) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-1066F | Mouse Anti-VIM Recombinant Antibody (clone NKI-M9 (former PAF2a)) | IF, IHC-Fr, IP | Mouse IgG2a |
| ZG-1102F | Mouse Anti-VIM Recombinant Antibody (clone RV204) | IHC, ICC, IB | Mouse IgG1 |
| ZG-1103F | Rabbit Anti-VIM Recombinant Antibody (clone RV205) | IHC, ICC, IB | Rabbit IgG |
| ZG-1113F | Mouse Anti-VIM Recombinant Antibody (clone V9) | FC, ICC, IHC-Fr, IHC-P, WB | Mouse IgG1 |
| VS3-QX1236 | Mouse Anti-VIM Recombinant Antibody (clone 4F8-A5-H5) | WB, IF, IP | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1237 | Mouse Anti-VIM Recombinant Antibody (clone 280CT3.4.6) | WB | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY274 | AbPlus™ Anti-VIM Magnetic Beads (NKI-M9) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-FY193 | Mouse Anti-VIM (clone HoAKs-1) scFv-Fc Chimera | WB, IHC, FC | Mouse IgG1, scFv-Fc |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: Neut, ELISA, IF, IP, FuncS, FC, ICC
Application: Neut, ELISA, FuncS
Application: ELISA, FC, IF, WB
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA, FuncS
Application: ELISA, FC, Neut, Inhib
-4.jpg)
Application: FC
Application: FC
Application: ELISA, Neut, FuncS
Application: WB, IHC-Fr, IHC-P, ICC, IF
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
