Mouse Anti-VTCN1 Recombinant Antibody (clone 278M1); scFv Fragment (CAT#: HPAB-N0405-YC-S(P))
This product is a Mouse scFv antibody fragment that recognizes Human VTCN1. FACS analysis demonstrated that 278M1 was capable of binding both human and mouse B7-H4.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/1356_C9_3_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/1356_C9_3_selected.jpg
Figure 2 IHC staining of human breast
(Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/ihc_selected.jpg
(Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/ihc_selected.jpg
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_9_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_8_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_4_6.jpg
Figure 6 Breast
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_B_3_4.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_B_3_4.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_7_8.jpg
(Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_7_8.jpg
Figure 8 RNA cell line category: Cell line enriched (SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134258-VTCN1
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134258-VTCN1
Specifications
- Host Species
- Mouse
- Type
- Mouse scFv
- Specificity
- Human VTCN1
- Species Reactivity
- Human, Mouse
- Clone
- 278M1
- Applications
- FC
Product Property
- Purification
- Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- See the COA
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Flow Cytometry.
Target
- Alternative Names
- B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 homolog 4; B7 superfamily member 1; T cell costimulatory molecule B7x; immune costimulatory protein B7-H4
- Gene ID
- 59272
- UniProt ID
- Q7Z7D3
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2216z | Mouse Anti-VTCN1 Recombinant Antibody (clone 21A2) | WB, ELISA | Mouse IgG1 |
| HPAB-0305-CN | Mouse Anti-VTCN1 Recombinant Antibody (clone 5F6) | ELISA, FC, IHC | Mouse IgG |
| HPAB-0306-CN | Mouse Anti-VTCN1 Recombinant Antibody (HPAB-0306-CN) | ELISA, FC, IHC, IF | Mouse IgG |
| HPAB-0335CQ | Human Anti-VTCN1 Recombinant Antibody (HPAB-0335CQ) | ELISA, FC | Human IgG1, κ |
| HPAB-0336CQ | Human Anti-VTCN1 Recombinant Antibody (HPAB-0336CQ) | ELISA, FC | Human IgG1, κ |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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