Anti-Human VTCN1 Recombinant Antibody (2A7)
CAT#: TAB-1122CL
The anti-B7-H4 antibiody specifically binds B7-H4 also known as O8E, the antibody can be used in the treatment of cancer, such as ovarian cancer, lung cancer and breast cancer.
Published Data
Gene Expression
ADCC
Figure 1 The results of antibody dependent cellular cytotoxicity (ADCC) assays demonstrating that human monoclonal anti-O8E antibodies kill O8E transfected SKOV3 cells in an ADCC dependent manner.
ADCC
Figure 2 The results of antibody dependent cellular cytotoxicity (ADCC) assays demonstrating that human monoclonal anti-O8E antibodies kill human breast cancer cell line SKBR3 in a concentration and ADCC dependent manner.
FuncS
Figure 3 The results of in vivo studies on SCID mice showing tumor growth inhibition of HEK-B7H4 tumors by anti-O8E antibodies.
FuncS
Figure 4 A graph showing the results of an in vivo HEK293-B7H4 xenograft mouse model, presenting median tumor volume in mice treated with vehicle alone, naked antibody, or antibody-partner molecule conjugates at various concentrations.
FuncS
Figure 5 A graph showing the results of an in vivo HEK293-B7H4 xenograft mouse model, presenting median body weight change in mice treated with vehicle alone, naked antibody, or antibody-partner molecule conjugates at various concentrations.
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Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane & cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/1356_C9_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human breast
Immunohistochemical staining of human breast shows moderate membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/ihc_selected.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_4_6.jpg
Normal Tissue
Figure 6 Breast
Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_B_3_4.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54200/147470_A_7_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (SK-BR-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000134258-VTCN1
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Specifications
- Host Species
- Human
- Type
- Fully human antibody
- Specificity
- Human
- Clone
- 2A7
- Applications
- ADCC, FuncS
- Related Disease
- Ovarian cancer, Lung cancer, Breast cancer
Applications
- Application Notes
- This antibody has been tested for use in Antibody-dependent Cellular Cytotoxicity Assay and Functional Assay.
Target
REVIEWS AND Q&AS
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Reliable research reagent
The Anti-Human VTCN1 Recombinant Antibody (2A7) has contributed significantly to our understanding of tumor immune evasion. It detected VTCN1 in our in vitro models and proved to be a reliable study reagent.
Excellent clarity
Our research focuses on immune checkpoint proteins, and the Anti-Human VTCN1 Recombinant Antibody (2A7) provided excellent clarity in detecting VTCN1 interactions. The antibody's high affinity was evident in all our experimental protocols.
Applicable to various cancer cell lines
We've applied this antibody in a comparative study involving various cancer cell lines. It consistently identified VTCN1 expression levels, helping us correlate VTCN1 presence with cancer aggressiveness. A truly invaluable asset for our research.
Q&As
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What cell lines are most suitable for testing this antibody, and why?
A: The Anti-Human VTCN1 Recombinant Antibody (2A7) is best tested in cell lines that exhibit high amounts of VTCN1, such as B7-H4-positive ovarian carcinoma cells. These provide robust test conditions for determining binding efficiency.
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How was the epitope mapping conducted for the Anti-Human VTCN1 Recombinant Antibody (2A7)?
A: Epitope mapping for this antibody involved a combination of peptide scanning and X-ray crystallography. Detailed computational modeling and alignment with known VTCN1 structures further refined the identified binding site.
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What concentration range should be used for the Anti-Human VTCN1 Recombinant Antibody (2A7) in Western Blot analysis?
A: For Western Blot analysis, a concentration range of 0.1 to 1 µg/mL of the Anti-Human VTCN1 Recombinant Antibody (2A7) is recommended to achieve clear, specific bands with minimal background noise.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| HPAB-0307-CN | Mouse Anti-VTCN1 Recombinant Antibody (HPAB-0307-CN) | ELISA, FC, IHC, IF | Mouse IgG |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1101CL | Mouse Anti-VTCN1 Recombinant Antibody (TAB-1101CL) | ELISA, WB, FC, FuncS | Mouse IgG |
| TAB-1109CL | Mouse Anti-VTCN1 Recombinant Antibody (TAB-1109CL) | ELISA, FC | Mouse IgG2 |
| TAB-1111CL | Mouse Anti-VTCN1 Recombinant Antibody (TAB-1111CL) | ELISA, FC | Mouse IgG1 |
| TAB-1113CL | Mouse Anti-VTCN1 Recombinant Antibody (TAB-1113CL) | ELISA, FC | Mouse IgG4 |
| TAB-1115CL | Mouse Anti-VTCN1 Recombinant Antibody (TAB-1115CL) | ELISA | Mouse IgG4 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1102CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1102CL) | ELISA | Humanized IgG1, κ |
| TAB-1103CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1103CL) | ELISA | Humanized IgG1, κ |
| TAB-1104CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1104CL) | ELISA | Humanized IgG1, κ |
| TAB-1105CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1105CL) | ELISA | Humanized IgG1, κ |
| TAB-1106CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1106CL) | ELISA | Humanized IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1110CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1110CL) | ELISA, FC | Chimeric (Mouse/Human) IgG4 |
| TAB-1112CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1112CL) | ELISA, FC | Chimeric (Mouse/Human) IgG4 |
| TAB-1114CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1114CL) | ELISA, FC | Chimeric (Mouse/Human) IgG4 |
| TAB-1110CL-F(E) | Human Anti-VTCN1 Recombinant Antibody; Fab Fragment (TAB-1110CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
| TAB-1112CL-F(E) | Human Anti-VTCN1 Recombinant Antibody; Fab Fragment (TAB-1112CL-F(E)) | ELISA, FC | Chimeric (Mouse/Human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1116CL | Mouse Anti-VTCN1 Recombinant Antibody (TAB-1116CL) | ELISA | Mouse IgM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-1117CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1117CL) | ELISA, FC, FuncS | Human IgG |
| TAB-1118CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1118CL) | ELISA, FC, FuncS | Human IgG |
| TAB-1119CL | Human Anti-VTCN1 Recombinant Antibody (TAB-1119CL) | ELISA, FC, FuncS | Human IgG |
| TAB-1121CL | Anti-Human VTCN1 Recombinant Antibody (11G1) | ADCC | Fully human antibody |
| TAB-1123CL | Anti-Human VTCN1 Recombinant Antibody (2F9) | ADCC | Fully human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2275CQ | Rat Anti-Vtcn1 Recombinant Antibody (clone 188) | FC, FuncS, Neut | Rat IgG2b, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3857 | Rabbit Anti-VTCN1 Recombinant Antibody (clone DS3857AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0405-YC-S(P) | Mouse Anti-VTCN1 Recombinant Antibody (clone 278M1); scFv Fragment | FC | Mouse scFv |
| HPAB-N0406-YC-S(P) | Human Anti-VTCN1 Recombinant Antibody (clone 278M1 L2H2); scFv Fragment | FC, Inhib | Human scFv |
| HPAB-N0297-YC-S(P) | Mouse Anti-VTCN1 Recombinant Antibody (clone B7H4/1E10); scFv Fragment | ELISA, WB | Mouse scFv |
| HPAB-0583-FY-S(P) | Mouse Anti-VTCN1 Recombinant Antibody; scFv Fragment (HPAB-0583-FY-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-0584-FY-S(P) | Mouse Anti-VTCN1 Recombinant Antibody; scFv Fragment (HPAB-0584-FY-S(P)) | ELISA, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-N0405-YC-F(E) | Mouse Anti-VTCN1 Recombinant Antibody (clone 278M1); Fab Fragment | FC | Mouse Fab |
| HPAB-N0406-YC-F(E) | Human Anti-VTCN1 Recombinant Antibody (clone 278M1 L2H2); Fab Fragment | FC, Inhib | Human Fab |
| HPAB-0583-FY-F(E) | Mouse Anti-VTCN1 Recombinant Antibody; Fab Fragment (HPAB-0583-FY-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-1811-FY-S(P) | Human Anti-VTCN1 Recombinant Antibody; scFv Fragment (HPAB-1811-FY-S(P)) | ELISA | Human scFv |
| HPAB-1812-FY-S(P) | Human Anti-VTCN1 Recombinant Antibody; scFv Fragment (HPAB-1812-FY-S(P)) | ELISA | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY232 | CytoStream™ Mouse Anti-VTCN1 Recombinant Antibody (clone MIH43) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2478 | Anti-VTCN1 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC263 | Recombinant Anti-VTCN1 Vesicular Antibody, EV Displayed (VS-0425-YC263) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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| VS-0525-YC191 | Recombinant Anti-VTCN1 (AA 177-181 x AA 238-257) Biparatopic Antibody, Tandem scFv | ELISA, FC | Tandem scFv |
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