CytoStream™ Rabbit Anti-ZAP70 Recombinant Antibody (VS13-YC1237) (CAT#: VS13-YC1237)

• Immunogen
• Recombinant human ZAP70, aa 570-619.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human ZAP70

• Species Reactivity
• Human

• Applications
• WB, ICC, IF, IHC-P, FC, IP

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunocytochemistry (ICC): 1/50-1/200
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
  Flow cytometry (FC): 1/50-1/100
  Immunoprecipitation (IP): 1/10-1/50

• Alternative Names
• Zeta Chain Of T Cell Receptor Associated Protein Kinase 70; SRK; 0 KDa Zeta-Chain Associated Protein; Tyrosine-Protein Kinase ZAP-70; Syk-Related Tyrosine Kinase; EC 2.7.10.2;6 Zeta Chain Of T Cell Receptor Associated Protein Kinase 70kDa; Zeta Chain Of T-Cell Receptor Associated Protein Kinase 70; Zeta-Chain (TCR) Associated Protein Kinase 70kDa; Zeta-Chain Associated Protein Kinase, 70kD;70 KDa Zeta-Associated Protein; EC 2.7.10;6 ADMIO2; ZAP-70; IMD48; STCD; STD; TZK;

• Gene ID
• 7535

• UniProt ID
• P43403

• Long Name
• Zeta Chain Of T Cell Receptor Associated Protein Kinase 70

• Sequence Similarities
• Belongs to the protein kinase superfamily. Tyr protein kinase family. SYK/ZAP-70 subfamily.

• Cellular Localization
• Cytoplasm; Cell membrane

• Protein Refseq
• NP_001070.2; NP_997402.1

• Function
• Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates motility, adhesion and cytokine expression of mature T-cells, as well as thymocyte development. Contributes also to the development and activation of primary B-lymphocytes. When antigen presenting cells (APC) activate T-cell receptor (TCR), a serie of phosphorylations lead to the recruitment of ZAP70 to the doubly phosphorylated TCR component CD247/CD3Z through ITAM motif at the plasma membrane. This recruitment serves to localization to the stimulated TCR and to relieve its autoinhibited conformation. Release of ZAP70 active conformation is further stabilized by phosphorylation mediated by LCK. Subsequently, ZAP70 phosphorylates at least 2 essential adapter proteins: LAT and LCP2. In turn, a large number of signaling molecules are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation. Furthermore, ZAP70 controls cytoskeleton modifications, adhesion and mobility of T-lymphocytes, thus ensuring correct delivery of effectors to the APC. ZAP70 is also required for TCR-CD247/CD3Z internalization and degradation through interaction with the E3 ubiquitin-protein ligase CBL and adapter proteins SLA and SLA2. Thus, ZAP70 regulates both T-cell activation switch on and switch off by modulating TCR expression at the T-cell surface. During thymocyte development, ZAP70 promotes survival and cell-cycle progression of developing thymocytes before positive selection (when cells are still CD4/CD8 double negative). Additionally, ZAP70-dependent signaling pathway may also contribute to primary B-cells formation and activation through B-cell receptor (BCR).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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