Human Anti-ITGB2 (clone Hu23F2G) scFv-Fc Chimera
CAT#: VS-0425-FY138
Anti-ITGB2 scFv-FC targets the integrin beta-2 (ITGB2) subunit, which is crucial for leukocyte adhesion and immune response. The fusion of scFv with the Fc region improves its pharmacokinetics and stability. This product is aimed at researchers studying immune responses, inflammation, and hematological disorders, providing potential for therapeutic applications in modulating immune function.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16894/202_B8_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line EFO-21
Immunofluorescent staining of human cell line EFO-21 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16894/131_C9_1_blue_red_green.jpg
Subcellular Location
Figure 3 IF staining of human cell line BJ
Immunofluorescent staining of human cell line U-4 OS shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/16894/1842_G1_63_blue_red_green.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (THP-1, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000160255-ITGB2
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Specifications
- Immunogen
- The immunogen used to generate the 23F2G antibody was the human T cell line Hut 78.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human ITGB2
- Species Reactivity
- Human
- Clone
- Hu23F2G
- Applications
- Blocking, Flow Cytometry, Immunohistochemistry
- Related Disease
- Inflammatory disease
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- In a cell binding assay, humanized 23F2G antibody (at 1.01 μg/mL) bound to LFA-1 positive cells as efficiently as murine 23F2G antibody.
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- Integrin Subunit Beta 2
- Gene ID
- 3689
- UniProt ID
- P05107
- Long Name
- Integrin Subunit Beta 2
- Sequence Similarities
- Belongs to the integrin beta chain family.
- Cellular Localization
- Cell membrane, Membrane raft
- Post Translation Modifications
- Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters.
Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
Glycosylation at Asn50, Asn116, Asn212, Asn254, Asn501, and Asn642
Ubiquitination at Lys362, Lys379, Lys462, Lys479, Lys561, Lys658, Lys744, Lys755, and Lys765
Modification sites at PhosphoSitePlus
Glycosylation from GlyConnect ITB2_HUMAN (1415)
Glycosylation from GlyGen 12 sites, 52 N-linked glycans (5 sites), 1 O-linked glycan (1 site)
- Protein Refseq
- XP_006724064.1; XP_047296719.1; XP_054180443.1; NP_000202.3; NP_001120963.2
- Function
- Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4.
Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL.
Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen.
Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain.
Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain.
Integrin ITGAM/ITGB2 is also a receptor for factor X.
Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1.
Contributes to natural killer cell cytotoxicity.
Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils.
Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation.
Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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