Human Anti-ITGB2 (clone Hu23F2G) scFv-Fc Chimera (CAT#: VS-0425-FY138)

• Immunogen
• The immunogen used to generate the 23F2G antibody was the human T cell line Hut 78.

• Host Species
• Human

• Type
• Human IgG1, scFv-Fc

• Specificity
• Human ITGB2

• Species Reactivity
• Human

• Clone
• Hu23F2G

• Applications
• Blocking, Flow Cytometry, Immunohistochemistry

• Related Disease
• Inflammatory disease

• Purity
• >95% as determined by SDS-PAGE

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• In a cell binding assay, humanized 23F2G antibody (at 1.01 μg/mL) bound to LFA-1 positive cells as efficiently as murine 23F2G antibody.
  The optimal dilution concentration is determined by the experiment.

• Alternative Names
• Integrin Subunit Beta 2

• Gene ID
• 3689

• UniProt ID
• P05107

• Long Name
• Integrin Subunit Beta 2

• Sequence Similarities
• Belongs to the integrin beta chain family.

• Cellular Localization
• Cell membrane, Membrane raft

• Post Translation Modifications
• Both Ser-745 and Ser-756 become phosphorylated when T-cells are exposed to phorbol esters.
  Phosphorylation on Thr-758 (but not on Ser-756) allows interaction with 14-3-3 proteins.
  Glycosylation at Asn50, Asn116, Asn212, Asn254, Asn501, and Asn642
  Ubiquitination at Lys362, Lys379, Lys462, Lys479, Lys561, Lys658, Lys744, Lys755, and Lys765
  Modification sites at PhosphoSitePlus
  Glycosylation from GlyConnect ITB2_HUMAN (1415)
  Glycosylation from GlyGen 12 sites, 52 N-linked glycans (5 sites), 1 O-linked glycan (1 site)

• Protein Refseq
• XP_006724064.1; XP_047296719.1; XP_054180443.1; NP_000202.3; NP_001120963.2

• Function
• Integrin ITGAL/ITGB2 is a receptor for ICAM1, ICAM2, ICAM3 and ICAM4.
  Integrin ITGAL/ITGB2 is also a receptor for the secreted form of ubiquitin-like protein ISG15; the interaction is mediated by ITGAL.
  Integrins ITGAM/ITGB2 and ITGAX/ITGB2 are receptors for the iC3b fragment of the third complement component and for fibrinogen.
  Integrin ITGAX/ITGB2 recognizes the sequence G-P-R in fibrinogen alpha-chain.
  Integrin ITGAM/ITGB2 recognizes P1 and P2 peptides of fibrinogen gamma chain.
  Integrin ITGAM/ITGB2 is also a receptor for factor X.
  Integrin ITGAD/ITGB2 is a receptor for ICAM3 and VCAM1.
  Contributes to natural killer cell cytotoxicity.
  Involved in leukocyte adhesion and transmigration of leukocytes including T-cells and neutrophils.
  Triggers neutrophil transmigration during lung injury through PTK2B/PYK2-mediated activation.
  Integrin ITGAL/ITGB2 in association with ICAM3, contributes to apoptotic neutrophil phagocytosis by macrophages.
  In association with alpha subunit ITGAM/CD11b, required for CD177-PRTN3-mediated activation of TNF primed neutrophils.

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