Human Anti-SMAD7 scFv-Fc Chimera (VS-0325-FY202) (CAT#: VS-0325-FY202)
The anti-SMAD7 scFv-Fc antibody targets SMAD7, an inhibitory SMAD protein that regulates TGF-β signaling. This antibody combines scFv specificity with Fc stability, making it a valuable tool for studying SMAD7's role in modulating TGF-β pathways, immune response, and its potential as a therapeutic target in fibrosis and cancer.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/281_D12_4_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/281_D12_4_selected.jpg
Figure 2 IHC staining of human lung
(Immunohistochemical staining of human lung shows moderate nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/ihc_selected.jpg
(Immunohistochemical staining of human lung shows moderate nuclear positivity.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/ihc_selected.jpg
Figure 3 IHC staining of human urinary bladder
(Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/ihc_selected.jpg
(Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/ihc_selected.jpg
Figure 4 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_8_5.jpg
Figure 5 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_9_3.jpg
Figure 6 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_7_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_7_4.jpg
Figure 7 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_8_5.jpg
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_8_5.jpg
Figure 8 Skin
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_9_1.jpg
(Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_9_1.jpg
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101665-SMAD7
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101665-SMAD7
Specifications
- Immunogen
- Peptides derived from SMAD7
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human SMAD7
- Species Reactivity
- Human
- Applications
- ELISA, Immunofluorescence
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- SMAD Family Member 7; Mothers Against DPP Homolog 8; MAD Homolog 8; HSMAD7; MADH7; MADH8; MAD, Mothers Against Decapentaplegic Homolog 7 (Drosophila); MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 7; Mothers Against Decapentaplegic, Drosophila, Homolog Of, 7; SMAD, Mothers Against DPP Homolog 7 (Drosophila)
- Gene ID
- 4092
- UniProt ID
- O15105
- Long Name
- SMAD Family Member 7
- Sequence Similarities
- Belongs to the dwarfin/SMAD family.
- Cellular Localization
- Nucleus; Cytoplasm
- Post Translation Modifications
- Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity).
Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity).
Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation.
In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1.
Modification sites at PhosphoSitePlus
Modification sites at neXtProt
- Protein Refseq
- XP_047293465.1; XP_054174598.1; NP_001177750.1; NP_001177751.1; NP_001177752.1
- Function
- Human SMAD7 is an inhibitory SMAD protein that antagonizes TGF-beta signaling. It plays a role in regulating fibrosis and inflammation by preventing excessive activation of the TGF-beta pathway.
Recommended Products
Isotype Control
Secondary Antibody
Related Resources
TGF-β Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
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| MOB-4083z | Mouse Anti-SMAD7 Recombinant Antibody (clone 21B4) | WB, IP, IF, ELISA | Mouse IgG1, κ |
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| VS3-XY1437 | Mouse Anti-SMAD7 Recombinant Antibody (clone 3E3) | WB, IHC |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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