Hi-Affi™ Recombinant Rabbit Anti-SMAD7 Monoclonal Antibody (TH275DS)
CAT#: MOR-4761
Recombinant Rabbit anti-SMAD7 monoclonal antibody for WB, IF, ICC, FCM, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/281_D12_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lung
Immunohistochemical staining of human lung shows moderate nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_8_5.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_9_1.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101665-SMAD7
❮
❯
Specifications
- Immunogen
- Peptides corresponding to Human SMAD7 (aa 370-389, 343-355)
- Host Species
- Rabbit
- Type
- IgG
- Species Reactivity
- Human
- Clone
- TH275DS
- Applications
- Used for immunoassay techniques such as: Western Blot; Immunofluorescence; Immunocytochemistry; Flow Cytometry
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- > 95% as determined by both RP-HPLC and SDS-PAGE analysis.
- Format
- Liquid
- Buffer
- PBS, pH 7.2
- Preservative
- 0.09% sodium azide
- Storage
- Store at -20°C for long-term storage. Store at 4°C for up to one month. Avoid freeze/thaw cycles.
Applications
- Application Notes
- FC: 2-4 µg x 10^6 cells; ICC: 1-2 µg/ml; IF: 1-2 µg/ml; WB: 1-2 µg/ml
Target
- Alternative Names
- SMAD Family Member 7; Mothers Against DPP Homolog 8; MAD Homolog 8; HSMAD7; MADH7; MADH8; MAD, Mothers Against Decapentaplegic Homolog 7 (Drosophila); MAD (Mothers Against Decapentaplegic, Drosophila) Homolog 7; Mothers Against Decapentaplegic, Drosophila, Homolog Of, 7; SMAD, Mothers Against DPP Homolog 7 (Drosophila); Mothers Against Decapentaplegic Homolog 8; SMAD, Mothers Against DPP Homolog 7; Mothers Against DPP Homolog 7; MAD Homolog 7; SMAD 7; CRCS3; Smad7
- Gene ID
- 4092
- UniProt ID
- O15105
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for MOR-4761. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "SMAD7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4083z | Mouse Anti-SMAD7 Recombinant Antibody (clone 21B4) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1117CT | Recombinant Mouse anti-Human SMAD7 Monoclonal antibody (EML551) | IHC-P, WB | |
| VS3-XY1437 | Mouse Anti-SMAD7 Recombinant Antibody (clone 3E3) | WB, IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B025(A) | Recombinant Anti-human SMAD7 Intrabody [(D-Arg)9] | FACS, ICC, FuncS | vhh-(D-Arg)9 |
| IAB-B025(G) | Recombinant Anti-human SMAD7 Intrabody [+36 GFP] | ELISA, ICC, FuncS | vhh-(+36GFP) |
| IAB-B025(T) | Recombinant Anti-human SMAD7 Intrabody [Tat] | PCA, Neut, FuncS | vhh-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3325 | Hi-Affi™ Recombinant Rabbit Anti-SMAD7 Monoclonal Antibody (DS3325AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY202 | Human Anti-SMAD7 scFv-Fc Chimera (VS-0325-FY202) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2109 | Anti-SMAD7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6725 | Anti-Human SMAD7 Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: Inhib, Cyt
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, Inhib, FC
Application: ELISA, FC
Application: ELISA, FC, Inhib, FuncS
Application: FC
-2.png)
Application: ELISA
Application: ELISA, IHC, WB
Application: ELISA, Neut
Application: ELISA, Inhib, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
