Recombinant Anti-human SMAD7 Intrabody [(D-Arg)9]
CAT#: IAB-B025(A)
Recombinant anti-SMAD7 intrabody linked to (D-Arg)9 works within cells and directs against SMAD7, expressed in CHO cells. The intrabody relates to inhibito Smad7 expression or function for the preparation of a pharmaceutical composition for the prevention, amelioration or treatment of a disease of the central nervous system and/or diseases related and/or caused by said disease of the central nervous system.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm, nucleoli fibrillar center, cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/281_D12_4_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lung
Immunohistochemical staining of human lung shows moderate nuclear positivity.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28897/ihc_selected.jpg
Normal Tissue
Figure 3 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows moderate nuclear positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_7_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_A_8_5.jpg
Normal Tissue
Figure 8 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26212/54804_B_9_1.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (HDLM-2)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101665-SMAD7
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Specifications
- Immunogen
- human SMAD family member 7
- Host Species
- llama
- Type
- vhh-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Fluorescence-activated cell sorting; Immunocytochemistry; Functional studies
Product Property
- Purity
- >95% as judged by SDS-polyacrylamide gel electrophoresis
- Storage
- 4 °C, -20°C if preferred
Target
- Alternative Names
- SMAD7; SMAD family member 7; CRCS3; MADH7; MADH8; mothers against decapentaplegic homolog 7; hSMAD7; MAD homolog 8; mothers against DPP homolog 8; SMAD, mothers against DPP homolog 7; Mothers against decapentaplegic, drosophila, homolog of, 7; MAD (mother
- Gene ID
- 4092
- UniProt ID
- O15105
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Related Signaling Pathways
TGF-β Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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|---|---|---|---|
| MOB-4083z | Mouse Anti-SMAD7 Recombinant Antibody (clone 21B4) | WB, IP, IF, ELISA | Mouse IgG1, κ |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B025(G) | Recombinant Anti-human SMAD7 Intrabody [+36 GFP] | ELISA, ICC, FuncS | vhh-(+36GFP) |
| IAB-B025(T) | Recombinant Anti-human SMAD7 Intrabody [Tat] | PCA, Neut, FuncS | vhh-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3325 | Hi-Affi™ Recombinant Rabbit Anti-SMAD7 Monoclonal Antibody (DS3325AB) | WB | IgG |
| MOR-4761 | Hi-Affi™ Recombinant Rabbit Anti-SMAD7 Monoclonal Antibody (TH275DS) | WB, IF, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-FY202 | Human Anti-SMAD7 scFv-Fc Chimera (VS-0325-FY202) | ELISA, IF | Human IgG1, scFv-Fc |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2109 | Anti-SMAD7 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6725 | Anti-Human SMAD7 Immunohistochemistry Kit | IHC |
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