Mouse Anti-CD74 Recombinant Antibody (clone 2D1B11) (CAT#: VS3-XY404)
Clone 2D1B11 is a mouse antibody, which reacts with human CD74 protein. This antibody is suitable for CD74 detection by ELISA, WB, FC assays.
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Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/if_selected.jpg
Figure 2 IHC staining of human spleen
(Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
(Immunohistochemical staining of human spleen shows strong granular cytoplasmic positivity.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/10592/ihc_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
(Immunohistochemical staining of human tonsil shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79994/170659_A_6_8_selected.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/203_F11_1_blue_red_green.jpg
Figure 5 Colon
(Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
(Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity: 75%-25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_7_3.jpg
Figure 6 Kidney
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
(Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_5.jpg
Figure 7 Lymph node
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_8_8.jpg
Figure 8 Tonsil
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
(Germinal center cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2506/6593_A_6_8.jpg
Figure 9 RNA cell line category: Cell line enhanced (CAPAN-2, Daudi, HDLM-2, REH, RPMI-8226, U-266/70, U-698)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000019582-CD74
Specifications
- Immunogen
- Recombinant fragment of human CD74 (a.a. 1-106) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CD74
- Species Reactivity
- Human
- Clone
- 2D1B11
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Extracellular Vesicle (EV) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1542z | Mouse Anti-CD74 Recombinant Antibody (clone 24B6) | FC, IF, IHC, IP, FuncS | Mouse IgG1 |
| ZG-0135J | Mouse Anti-CD74 Recombinant Antibody (ZG-0135J) | IHC-P, IF | Mouse IgG |
| ZG-0136J | Mouse Anti-CD74 Recombinant Antibody (ZG-0136J) | IHC-P, IF | Mouse IgG |
| ZG-0594J | Rabbit Anti-CD74 Recombinant Antibody (clone 8H11) | ELISA, IHC, FC | Rabbit IgG |
| VS3-CJ338 | Mouse Anti-CD74 Recombinant Antibody (VS3-CJ338) | IHC | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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