Mouse Anti-RET Recombinant Antibody (clone 8D10C9)
CAT#: VS3-XY1369
Clone 8D10C9 is a mouse antibody, which reacts with RET protein. This antibody is suitable for RET detection by ELISA, WB assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to plasma membrane, cytosol & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8356/1081_D1_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human parathyroid gland
Immunohistochemical staining of human parathyroid gland shows strong positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_3_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_B_8_5.jpg
Normal Tissue
Figure 4 Parathyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_3_8.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: Not detected
Intensity: Weak
Quantity: <25%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2581/6496_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (MCF7, NB-4, SH-SY5Y, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000165731-RET/subcellular
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Specifications
- Immunogen
- Recombinant fragment of RET (a.a. 896-1063) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RET
- Species Reactivity
- Human
- Clone
- 8D10C9
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000) and Enzyme-linked Immunosorbent Assay (1/10000).
Target
- Alternative Names
- Ret Proto-Oncogene; Cadherin-Related Family Member 16; Rearranged During Transfection; RET Receptor Tyrosine Kinase; Cadherin Family Member 12; Proto-Oncogene C-Ret; EC 2.7.10.1; CDHF12; CDHR16; PTC; Ret Proto-Oncogene (Multiple Endocrine Neoplasia And Medullary Thyroid Carcinoma 1, Hirschsprung Disease)
- Gene ID
- 5979
- UniProt ID
- P07949
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Related Diseases
EGFR Tyrosine Kinase Inhibitor Resistance
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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